PPT-RECOMBINANT DNA Introduction

Definition Steps Applications INTRODUCTION Recombinant DNA   rDNA   DNA  molecules formed by laboratory methods of  genetic recombination  such as  molecular cloning to bring together genetic material from multiple sources creating . Recombinant DNA Technology. T. echnique that allows DNA to be . combined. from different sources. DNA code universal. Foundation of genetic engineering. Review. What are enzymes? What function do they serve?. Objective. Students will model the process of using restriction enzymes and plasmids to form recombinant DNA.. Background Information. major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes.. Will Herrick. Peyton Group Meeting. March 20, 2013. What Are Adenoviruses?. Linear . dsDNA. virus. 80-100 nm. Nonenveloped. Icosahedral structure:. 1: penton . capsomeres. 2: . hexon. . capsomeres. Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Brief Chapter Outline.  . I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy. Cutting . and Joining DNA. DNA . Cloning. V. Cloning Vectors. A. . Bacterial Vectors. DNA damage DNA gets damaged a lot ! DNA damage DNA gets damaged a lot! >200,000 events/human cell/day DNA damage Occurs 2 ways 1) spontaneously 2) mutagens : damage DNA Transposons Discovered by Barbara McClintock A deadly pathogen called the papaya . ringspot. virus (PRV) had spread throughout the islands. . It appeared poised to completely decimate the papaya plant population.. Scientists from the University of Hawaii were able to rescue the industry by creating new, genetically engineered PRV-resistant strains of papaya.. BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by the human gene.. Technology AQC - 321 Dr. Mamta Singh Assistant Professor COF (BASU), Kishanganj Recombinant DNA Technology... Definition : It is a technology of joining together of DNA molecules from two different s 683 Human Erythropoietin (hEPO) is the main hormone involved in the differentiation, proliferation and maintaining of physiologic levels of erythroid stem cells, it was also the rst hematopoietic great promise, and several types are being tested in. Humans. • DNA vaccines take immunization to . a new. technological. level. • . These vaccines dispense with both the whole . organism and . Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . Profit. What the heck is recombinant DNA?. Recombinant DNA is what you get when you combine DNA from two different sources.. For Example:. Mouse + Human DNA. Human + Bacterial DNA. Viral + Bacteria DNA.