PPT-Recombinant DNA technology

Recombinant DNA technology Methods used to join together recombine different DNA segments that are not found together in nature This technique is used in genetic analysis to serve several applications. PLASMID VECTORS. . . Cloning into a Plasmid. Bacteria are useful hosts.. They are easily grown. They are cheap to grow. They grow fast. They are easily manipulated in the laboratory. DNA can be inserted - transformation. Recombinant DNA Technology. T. echnique that allows DNA to be . combined. from different sources. DNA code universal. Foundation of genetic engineering. Review. What are enzymes? What function do they serve?. Will Herrick. Peyton Group Meeting. March 20, 2013. What Are Adenoviruses?. Linear . dsDNA. virus. 80-100 nm. Nonenveloped. Icosahedral structure:. 1: penton . capsomeres. 2: . hexon. . capsomeres. Recombinant . DNA technology. Methods . used to join together (recombine) different DNA segments that are not found together in nature.. This technique is used in genetic analysis to serve several applications:. Introduction. Lecture 1. Biotechnology. It implies with the use of microorganisms, plants, animals or parts of them for the production of useful compounds.. Pharmaceutical biotechnology. It is concerned as the biotechnological manufacturing of pharmaceutical products. . Brief Chapter Outline.  . I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy. Cutting . and Joining DNA. DNA . Cloning. V. Cloning Vectors. A. . Bacterial Vectors. DNA damage DNA gets damaged a lot ! DNA damage DNA gets damaged a lot! >200,000 events/human cell/day DNA damage Occurs 2 ways 1) spontaneously 2) mutagens : damage DNA Transposons Discovered by Barbara McClintock A deadly pathogen called the papaya . ringspot. virus (PRV) had spread throughout the islands. . It appeared poised to completely decimate the papaya plant population.. Scientists from the University of Hawaii were able to rescue the industry by creating new, genetically engineered PRV-resistant strains of papaya.. www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Construction & Cloning of Recombinant DNA. From Campbell Biology, . Chp. . 20. Recombinant DNA is a technology scientists developed that made it possible to insert a human gene into the genetic material of a common bacterium. This “recombinant” micro-organism could now produce the protein encoded by the human gene.. great promise, and several types are being tested in. Humans. • DNA vaccines take immunization to . a new. technological. level. • . These vaccines dispense with both the whole . organism and . Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . Dr.V. S. Bheemareddy. Following . steps are involved in genetic engineering. .. Isolation of desired gene or DNA.. Construction of recombinant DNA. Insertion of recombinant DNA in to host cells. Selection of transformed cells or organisms. Profit. What the heck is recombinant DNA?. Recombinant DNA is what you get when you combine DNA from two different sources.. For Example:. Mouse + Human DNA. Human + Bacterial DNA. Viral + Bacteria DNA.