to Platelet Serotonin Platelet rich plasma Expected fixed platelet count Unstable ACS environment Potential confounders Offsite laboratory processing delayed time to processing Platelet Serotonin Laboratory Protocol ID: 206271
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Study Limitations Related
to Platelet Serotonin
Platelet
-rich plasma
Expected fixed platelet count
Unstable ACS environment
Potential
confounders
Off-site laboratory processing→
delayed time to processingSlide4
Platelet Serotonin Laboratory Protocol
Remove 400
uL
of plasma
Add to 15 mL tube for platelet purification
Add 1.5 mL physiological saline to platelet-rich plasma (PRP)
Centrifuge 10 minutes at 200 RCF(xg) at ambient temperature
Centrifuge10 minutes at 4,500 RCF(xg) at 4
o
C & discard supernatant
Add to 300 uL distilled water
Vortex pellet until the platelets are mixed & transfer 300 uL to 1 aliquot
Transfer to -80
o
C freezer for storage
ELISA
Immuno Biological Laboratories (2009). Serotonin ELISA IB89546. [Laboratory instructions]. IBL-America
4 mL EDTA tubeSlide5
Storage temperature
Duration of storage
Freeze-thaw cycles
Specimen
Storage
I
nstrument calibration & controls
Time between collection & centrifugation
Vortex technique
Centrifugation
Type
Calibrated
Temperature
Speed
Duration
Automated decapping
Specimen separated into aliquots
Aliquot labeling
Processing
In Vivo Haemolysis due to patient condition
Proper labeling
Mode of collection
Needle gauge
Position of arm
Location of venipuncture
Antiseptic used
Tourniquet time
Syringe transfer
Specimen tube
Type
Under/over filled
Order of draw
Under/over mixing
Phlebotomy
Origin of specimen
Vertical or horizontal transportation of tube
Pneumatic tube transport
Courier transportTransportation durationCentrifugation prior to transferTransfer temperature
SpecimenTransport
Factors Affecting Biological Specimens