Faculty Of Pharmacy Department Of Pharmacology and Clinical Pharmacy Selenium effect on infertility induced by formaldehyde using male albino mice Suhera M Aburawi 1 Habiba A El jaafari ID: 555183
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University Of TripoliFaculty Of Pharmacy
Department Of Pharmacology and Clinical Pharmacy
Selenium effect on infertility induced by formaldehyde using male albino mice
Suhera M. Aburawi1, Habiba A. El jaafari2, Soad A. Treesh3, Abdulssalam M. Abu-Aisha1, Faisal S. Alwaer1, Reda A. Eltubuly1, Medeha Elghedamsi11, Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy; 2, Zoology Department, Science Faculty; 3, Department of Histology and medical genetics, Faculty of Medicine - University of Tripoli Slide2
Male Infertility
Means that men are not able to conceive a child even through they have had frequent.
Male Infertility is due to low sperm
production, misshapen or immobile sperm or blockages that prevent the delivery of sperm (McPhee et al., 2012).is the inability of a sexually active, non-contracepting couple to achieve pregnancy in one year (
WHO, 2000
).
Infertility
is a source of psychological, and sometimes social, stress on parents who desire to have children.Slide3
Male Reproductive Toxicity of Formaldehyde
Formaldehyde is an important precursor to many other materials and chemical compounds (
Reuss
et al., 2002).The medical uses of formaldehyde are limited, but focused especially on laboratory use (Zahra, 2007). High dose of formaldehyde resulted in pathological changes in the seminiferous tubules ( STs ) of adult rats and mice (Tang et al., 2003 ).Slide4
Selenium
is an essential trace mineral element for human and animals. Is found in soil, rocks and consequently may then accumulate in plants (
Yang and Zhou, 1994
).naturally present in some foods, such as : Fish, Shellfish, Red meat, Offal, Chicken, Liver, Garlic, Eggs, Brazil Nuts and Grains (Escott-Stump, 2008).plays an important role in a number of biological processes (Combs and Combs, 1986). Slide5
act by trapping free oxygen radicals and scavenging and converting free radicals into stable compounds
(
Sieja
and Talerezyk, 2004).
is present in
selenoenzymes
, such as glutathione peroxidase and
thioredoxin
reductase
, which indirectly reduce certain oxidized molecules and protect DNA and other cellular components from oxidative damage
(
Valko
, et al., 2006
).
is essential for sperm function and male fertility. Selenium deficiency has been linked to reproductive problems in rats, mice, chickens, pigs, sheep, and cattle (
Combs and Combs, 1986
).Slide6
Goal of the work
To investigate the
e
ffect of selenium on male infertility induced by formaldehyde using male albino miceSlide7
Treatment
groups
N = 8
Day 1Day 2
Day 3
Day 4
Day 5
Day 6
Group I
Water
for
injection (
5ml/day)
Water
for injection (5ml/day)Water for injection (5ml/day)Water for injection (5ml/day)Water for injection (5ml/day)ScoringGroup II Selenium100μg/kgSelenium100μg/kgSelenium100μg/kgSelenium100μg/kgSelenium100μg/kgScoringGroup III (Peng et al., 2006)Formaldehyde30 mg/kgFormaldehyde30 mg/kgFormaldehyde30 mg/kgFormaldehyde30 mg/kgFormaldehyde30 mg/kgScoringGroup IV (prophylaxis)Formaldehyde+ SeleniumFormaldehyde+ SeleniumFormaldehyde+ SeleniumFormaldehyde+ SeleniumFormaldehyde+ SeleniumScoringGroup V( curative)FormaldehydeFormaldehydeFormaldehydeFormaldehydeFormaldehydeSeleniumSeleniumSeleniumSeleniumSeleniumSeleniumScoring
Design of the workSelenium effect on infertility induced by formaldehyde using male albino miceSlide8
Sperm Counting
Counting of mice sperm is done by Hematocytometer slide.Slide9
Results and DiscussionSlide10Slide11Slide12Slide13Slide14Slide15Slide16Slide17Slide18Slide19Slide20
Formaldehyde
In formaldehyde reproductive toxicity, there were more male than female exposure studies.
This may be because effects on
male reproduction are more readily observable and require fewer invasive procedures (
Duong et al., 2011
)
.Slide21
Formaldehyde significantly
decreased
the activities of superoxide dismutase and glutathione
peroxidase (Shi et al., 2012).Reactive oxygen species-mediated oxidative damage resulting from formaldehyde exposure has been detected in reproductive tissues (Im et al., 2006). Formaldehyde is genotoxic, inducing:Chromosomal aberrations (Natarajan
et al., 1983
).
DNA breakage (
Wang et al., 2006a
).
DNA-protein
crosslinks
(
Peng
et al., 2006; Wang et al., 2006c
).Mediated aberrant DNA methylation which has been associated with male gametogenic defects (Navarro-Costa et al., 2010). Slide22
Lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) are involved in the
maturation of spermatogenic cells, testis and spermatozoa and with energy metabolism of spermatozoa.
Formaldehyde
decrease the activity of SDH in mice testicular (Xie et al., 2003). SDH activity was positively correlated with sperm cell counts, and negatively correlated with the abnormal rate of sperm heads (Tang et al., 2003).Slide23
Formaldehyde exposure
decreased serum testosterone levels were in male mice and rats (
Chowdhury et al., 1992; Ozen
et al., 2005; Zhou et al., 2006), to leading to disruption of male reproductive function.Formaldehyde treatment activates necrosis factor-κB (NF-κB) (
Shi et al., 2012
)
.Slide24
Selenium
There is significant
positive
correlations in selenium concentration between different reproductive organs, (Oldereid et al., 1998)Testis having the highest concentrations.In selenium-deficient rats, flagellar disorganization during spermiogenesis and post-testicular sperm development were observed. loss of male fertility in selenium deficiency results from the sequential development of sperm defects
expressed during both spermiogenesis and maturation in the
epididymis
(
Olson et al., 2004
). Slide25
Selenium(
Shi et al., 2012)
counteracted formaldehyde-induced oxidative stress
,ameliorated DNA–protein cross-links and attenuated the activation of necrosis factor –κB.Slide26
ConclusionSlide27
Formaldehyde
toxicity
Sperm count is not changed
Percentage of motile sperm was not changed.Unhealthy sperm was increased, while healthy sperm was decreased.Formaldehyde produces degeneration/damage to male mice genital system.Slide28
Selenium
alone produce the following compared to healthy group
Sperm count is increased
.Volume of seminal fluid is larger .Percentage of motile sperm is increased.Selenium has prophylactic and curative effects against formaldehyde-induce genital system toxicity.
Future workSlide29
.
Chowdhury AR, Gautam AK, Patel KG,
Trivedi HS. (1992). Steroidogenic inhibition in testicular tissue of formaldehyde exposed rats. Indian J Physiol
Pharmacol.; 36:162–168. Combs GF, Combs BS. (1986). The Role of Selenium in Nutrition. London: Academic Press; 206-312.Duong A., Steinmaus C., McHale C.M., Vaughan C.P., Zhang L., (2011). Mutat Res.; 728(3): 118–138. Escott-Stump S, ed. Nutrition and Diagnosis-Related Care. 6th ed. Philadelphia, Pa: Lippincott Williams & Wilkins; 2008.Im H, Oh E, Mun J, Khim JY, Lee E, Kang HS, Kim E, Kim H, Won NH, Kim YH, Jung WW, Sul D. (2006). Evaluation of toxicological monitoring markers using proteomic analysis in rats exposed to formaldehyde. J Proteome Res.; 5:1354–1366.McPhee SJ, et al. Current Medical Diagnosis & Treatment 2012. 51st ed. New York, N.Y.: The McGraw-Hill Companies. http://www.accessmedicine.com/resourceTOC.aspx?resourceID=1. Accessed July 20, 2012Natarajan AT,
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F,
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CJ, van
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-van
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AC. (1983). Evaluation of the
mutagenicity
of formaldehyde in mammalian cytogenetic assays in vivo and vitro.
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Thank You
…