PPT-Chapter 9 Control of Microbial Growth

Ignaz Semmelweis and Joseph Lister developed the first microbial control methods Mid 1800s Before aseptic surgical techniques 10 of all deaths were due to nosocomial infections Delivering mothers as high as 25 death rate. And 57375en 57375ere Were None meets the standard for Range of Reading and Level of Text Complexity for grade 8 Its structure pacing and universal appeal make it an appropriate reading choice for reluctant readers 57375e book also o57373ers students 1 Microbial Growth:4Refers to an increase in cell , not in cell size.4Bacteria grow and divide by binary a rapid and relatively simple process. Requirements for GrowthPhysical Requirements1. : Microb in vitro. CLS 212: Medical Microbiology. . Microbicidal. or . Microbistatic. ?. . Microbicidal. . Microbicidal. . is . the process . or . an agent . that . kills. the microorganism. . A. Metabolism. 1. Anabolism. A) synthesis reactions (building up). 2. Catabolism. A) lysis reactions (breaking down). B. Enzymes. 1. proteins that facilitate chemical reactions. Microbial Metabolism. Eric J. Raes, P.E. ., LSRP. Engineering and Land Planning . Dora Ogles, Brett Baldwin, Kate Clark & . Anita Biernacki. Microbial Insights Inc.. Kerry Sublette, Ph.D., Kate Key. University of Tulsa. THE ORIGIN AND EVOLUTION OF MICROBIAL LIFE. HOW DID LIFE ORIGINATE?. SPONTANEOUS GENERATION. LIFE ARISING FROM NON-LIVING MATTER. LONG BELIEVED AS THE ACCEPTED EXPLANATION OF ORIGINATION OF LIFE. EXPERIMENTS EVENTUALLY SHOWED IT TO BE IMPOSSIBLE. A. Microbial Reproduction. 1. binary (transverse) fission. A) parent cell enlarges and duplicates all its genetic material. B) DNA copies move to opposite ends of parent and attach to a section of the cell membrane as it begins to pinch together at the center. A. Structure of DNA. 1. discovered by James Watson and Francis Crick in 1953. 2. stores genetic information for the production of proteins. 3. composed of nucleotides. A) a nucleotide is composed of a nitrogenous base, sugar, and phosphate. . Definitions. Indigenous . flora. microorganisms . native or belonging naturally to a region.. Colonization. establishment . of a site of reproduction of microbes on a person without necessarily resulting in tissue invasion or . Microbiology. Microbial Growth. Microbial requirements for growth:. 1. . Physical. A. . Temperature. Optimal growth temperature. Permissible range. human pathogens optimal = . 37°C. Microbial Growth. (a) Intrinsic factors: . These. . are inherent in the food. They include:. pH,. . water activity,. . oxidation reduction potential,. . nutrient content,. . antimicrobial contents,. . biological structure. Growth: . A. n . increase in cell number, not in cell size. .. Bacteria . grow and divide by binary fission, a rapid and relatively simple process. .. Phases . of Growth Bacterial Growth Curve : . When . 1. Microbial survival strategies. 2. Microbial growth. 3. Effects of the environment. 4. Microbial cultures. 5. Growth measurements. 6. Antimicrobial agents. 7. Antibiotics. 1. . Microbial. . survival. 1. Temperature. 2. . PH. 3. . Water activity. 4. . Oxygen and redox. Temperature. Minimum Temperature. . The temperature below which no growth occurs.. At temperature below the minimum the properties of cell membranes change so that they can no longer transport materials into the cell.