PPT-Microbial growth Microbial

Growth A n increase in cell number not in cell size Bacteria grow and divide by binary fission a rapid and relatively simple process Phases of Growth Bacterial Growth Curve When . A. Metabolism. 1. Anabolism. A) synthesis reactions (building up). 2. Catabolism. A) lysis reactions (breaking down). B. Enzymes. 1. proteins that facilitate chemical reactions. Microbial Metabolism. Microbial food spoilage occurs as a consequence of either microbial growth in a food or release of extracellular and intracellular(following cell . lysis. ) . enzymes in the food environment.. Signs of spoilage of different types of food:. Batch culture and Kinetics of Microbial growth in batch culture. After inoculation the growth rate of the cells gradually increases.. The cells grow at a constant, maximum, rate and this period is known as the . Eric J. Raes, P.E. ., LSRP. Engineering and Land Planning . Dora Ogles, Brett Baldwin, Kate Clark & . Anita Biernacki. Microbial Insights Inc.. Kerry Sublette, Ph.D., Kate Key. University of Tulsa. THE ORIGIN AND EVOLUTION OF MICROBIAL LIFE. HOW DID LIFE ORIGINATE?. SPONTANEOUS GENERATION. LIFE ARISING FROM NON-LIVING MATTER. LONG BELIEVED AS THE ACCEPTED EXPLANATION OF ORIGINATION OF LIFE. EXPERIMENTS EVENTUALLY SHOWED IT TO BE IMPOSSIBLE. Ignaz. . Semmelweis. and Joseph Lister developed the first microbial control methods.. Mid 1800s. Before aseptic surgical techniques ~10% of all deaths were due to nosocomial infections. Delivering mothers as high as 25% death rate. A. Metabolism. 1. Anabolism. A) synthesis reactions (building up). 2. Catabolism. A) lysis reactions (breaking down). B. Enzymes. 1. proteins that facilitate chemical reactions. Microbial Metabolism. . Definitions. Indigenous . flora. microorganisms . native or belonging naturally to a region.. Colonization. establishment . of a site of reproduction of microbes on a person without necessarily resulting in tissue invasion or . In the Environment. Table 9.1 Terminology of Microbial Control. Figure 9.1 A plot of microbial death rate.. Basic Principles of Microbial Control. Action of Antimicrobial Agents. Alteration of cell walls and membranes. Updated: January 2015. By Jerald D. . H. endrix. Microbial Classification . and Taxonomy. Classification Systems. Levels of Classification. Definition of “Species”. Nomenclature. Useful Properties in Microbial Classification. Microbiology. Microbial Growth. Microbial requirements for growth:. 1. . Physical. A. . Temperature. Optimal growth temperature. Permissible range. human pathogens optimal = . 37°C. Microbial Growth. (a) Intrinsic factors: . These. . are inherent in the food. They include:. pH,. . water activity,. . oxidation reduction potential,. . nutrient content,. . antimicrobial contents,. . biological structure. Musafer. PhD. Microbial growth defined as an increase in cellular constituents resulting an increase in a microorganism size, population number or both.. 1-Lag phase. When M.O are inoculated into fresh culture medium, will not reproduce immediately, therefore this period is called the lag phase. . . Kinetics. Growth Curve. Log CFU/ml. Optical Density. Lag. First Order Kinetics. Food microbiology is concerned with all phases. Of microbial growth (. lag,log. , stationary, death phase).Growth curves are normally plotted as the number of cells on a log scale or log10 cell number versus time..