Handson session alpha amylase alpha amylase as a saliva based biomarker for the analysis of stress in a motivational fitsituation Most previous ID: 1034360
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1. Belgrad, 23.10.2015Dr. Sebastian Schwab
2. Hands-on session: alpha amylasealpha amylase as a saliva based biomarker for the analysis of stress in a motivational fit-situation
3. Most previous studies demonstrate that a regulatory fit in motor and cognitive tasks will lead to performance improvements.What could be the reason for that?Which mechanism could be behind it?Is it a neurobiological mechanism? stress???problem
4. Edwards et al., 2007, Work & Stress; Wallace et al., 2009, Journal of Applied Psych.Plessner et al., 2009, Psych. of Sport and Exercise; Memmert et al., 2009, Zeitschrift für Sportpsych.; Shah et al., 1998, Journal of Personality and Soc. Psych.; Keller & Bless, 2006, Euro. Journal of Soc. Psych.;Spiegel et al., 2004, Euro. Journal of Soc. Psych.Is stress as a physiological parameter a potential mediator to explain the better performances in motivational fit-situations? motivationperformancestress
5. The participants‘ stress level in fit-situations remains constant or rather decreases, whereas under non-fit-conditions an increase of the stress activity will be observed.hypothesis
6. letter of agreementpersonal questionnairequestionnaire(Keller & Bless, 2006, Euro. J. Soc. Psychol.)(Friedman & Förster, 2001, J. Perso. and Soc. Psychol.)(Friedman & Förster, 2001)(Friedman & Förster, 2005, J. Perso. and Soc. Psychol.; Friedman et al., 2003)
7. alpha amylase is an index for stress-induced activity of the sympathetic nervous system (SNS) (Rohleder & Nater, 2009, Psychoneuroendocrinology) non-invasive with a saliva sample responds to stress immediately and without great time delay in comparison to cortisol (Strahler, 2012) also applicable in possible field studies apeears to be a reliable, simple, saliva based indirect marker of the SNS-activity (Nater & Rohleder, 2009, Psychoneuroendocrinology; Rohleder & Nater, 2009, Psychoneuroendocrinology)
8. (Rohleder et al., 2004, Ann. N.Y. Acad. Sci.; Chatterton et al., 1996, Clin. Physiol.)alpha amylase correlates with (nor-)adrenalin
9. alpha amylase – sampling methodsdroolingspittingsucitionabsorptionRohleder and Nater (2009) summarized that for saliva sampling, salivettes and passive drooling are both equally well suited.
10. alpha amylase – storageup to 3 weeksup to 12 monthsup to 5 months
11. alpha amylase – analysisProf. Dr. C. KirschbaumBiopsychology - TU DresdenZellescher Weg 19, Raum 212D-01062 Dresden, GermanyPack salivettes in zipper bags
12. briefingWhat are the methodic characteristics when measuring alpha amylase?Which are the influencing factors of alpha amylase?
13. alpha amylase – daily fluctuations(Rohleder et al., 2004, Ann. N.Y. Acad. Sci.)
14. alpha amylase – age(Strahler et al., 2010, Psychophysiol.)
15. alpha amylase – caffeine(Klein et al., 2010, Hum. Psychopharmacol. Clin. Exp.)
16. alpha amylase – food(Fröhlich et al., 1987, Physiol. Behav.)
17. alpha amylase – gender(van Stegeren et al., 2008, Int. J. Psychophysiol.)
18. alpha amylase – exercise(Sariri & Damirchi, 2010, Pharmacologyonline)
19. methodic conclusionsdaily fluctuationsagecaffeinefoodexercisegendertest between 10 and 12 o‘clocksport studentsno coffeeno big mealno exercisemen
20. (Schwab, Wolf & Memmert, 2015, Stress)non-fit fit64 male students between 20 and 33 years (M = 24.29, SD = 3.123)r = -0.249, p < .05
21. task enjoyment: r = 0.181, p = .076 (clear trend)motivation: r = 0.219, p < .05 Participants are more motivated when being in a relative fit-situation compared to a relative non-fit-situation. more results
22. 64 male students between 20 and 33 years (M = 24.29, SD = 3.123)Diff_1_2: t(1,58) = -2.540, p < .05 Diff_1_3: t(1,58) = -2.278, p < .05(Schwab, Wolf & Memmert, 2015, Stress)
23. discussion Our data indicate that the SNS was influenced by a motivational task: sAA activity of participants in a fit-situation decreased, while it increased in a non-fit-situation.Differential effects on the SNS could be seen as a possible mediator to explain the benefits of a fit-situation in contrast to a non-fit-situation.
24. future prospects Future research designs should try to replicate these findings with the help of cognitive and motor performance tasks that have already been used under rgulatory fit-aspects.With different tasks and questionnaires, it would be possible to evaluate whether participants under fit-conditions perform better than participants under non-fit-conditions, along with determining sAA activity of participants in a fit-situation decreases versus that of participants in a non-fit-situation.
25. Thank you for your attention!s.schwab@dshs-koeln.de