DNA Cleanup Tucson High School Biotechnology Course Spring 2010 What have we done so far psbA DNA pol g23 negative control virus DNA positive control negative control virus DNA positive control ID: 1045600
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1. Gel Interpretation&DNA Clean-upTucson High SchoolBiotechnology CourseSpring 2010
2. What have we done so far?psbADNA polg23negative controlvirus DNApositive controlnegative controlvirus DNApositive controlnegative controlvirus DNApositive control
3. What samples should we see a band for?psbADNA polg23negative controlvirus DNApositive controlnegative controlvirus DNApositive controlnegative controlvirus DNApositive controlNONONOYESYESYESMAYBEMAYBEMAYBE
4. Interpreting your gelpsbADNApolg23Negative controlPositive controlVirusNegative controlPositive controlVirusNegative controlPositive controlVirus
5. What should you write about your gel?
6. MyovirusPodoviruspsbADNA polg23XXXXHypothesis
7. Next… SequencingNeed to Clean Up DNAPCR reactiontaq polymerasedNTPsprimersMgCl2bufferDNADNAclean up
8. MinElute pcr Clean-Uppcr productsBuffer PBMinEluteColumnMinEluteColumnBuffer PEMinEluteColumnBuffer EBKEEP!!
9. Which pcr products will you clean up?psbADNApolg23Negative controlPositive controlVirusNegative controlPositive controlVirusNegative controlPositive controlVirus