Goldsmith CS Ksiazek TG Rollin PE Comer JA Nicholson WL Peret T et al Cell Culture and Electron Microscopy for Identifying Viruses in Diseases of Unknown Cause Emerg Infect Dis 2013196886891 httpsdoiorg103201eid1906130173 ID: 1046896
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1. Figure 1Figure 1. . . A) Cell culture isolate of severe acute respiratory syndrome coronavirus, in which virions are seen in the cisternae of the budding compartment (arrow). Also present are an inclusion of viral nucleocapsids (arrowhead) and double-membrane vesicles (asterisk). Scale bar = 100 nm. B) Coronavirus particles in cytoplasmic vesicles that appear to migrate to the cell surface. Virions are seen lining the cell membrane (arrow), a characteristic feature of this virus. Scale bar = 500 nm. C) Large, pleomorphic, extracellular Nipah virus particles (arrow), in which the viral envelope encloses the nucleocapsids. Scale bar = 500 nm. D) Nipah virus nucleocapsids (arrow) aggregate in the cytoplasm and become tightly apposed to the cell membrane as the virus begins the process of budding. Scale bar = 100 nm.Goldsmith CS, Ksiazek TG, Rollin PE, Comer JA, Nicholson WL, Peret T, et al. Cell Culture and Electron Microscopy for Identifying Viruses in Diseases of Unknown Cause. Emerg Infect Dis. 2013;19(6):886-891. https://doi.org/10.3201/eid1906.130173