Laboratory Laboratory Health Cooperation and Duties Why are we here To make the lab function more efficiently To address issues that reinforce those stated in Laboratory Safety training To promote good practices and aseptic techniques for proper health limited contamination and coopera ID: 186739
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Slide1
Genco Laboratory
Laboratory Health
Cooperation andDutiesSlide2
Why are we here?
To make the lab function more efficiently.
To address issues that reinforce those stated in Laboratory Safety training.To promote good practices and aseptic techniques for proper health, limited contamination and cooperation in the laboratory.To keep Eve away.Slide3
Laboratory Issues to Discuss
General
Laboratory CleanlinessOrganization and CourtesyOrdersLaboratoryEquipment
Biosafety HoodsLaboratory CoatsWaste Disposal and Dishes
Laboratory DutiesSlide4
Laboratory Cleanliness
Let’s keep things clean! How?
Keep the lab clean by putting things back in an orderly fashionie chemicals and reagents.Do not leave at your desk, next to the scale etc.
Clean any messes you make or find. If you spill something on any of the instruments or the floor, clean it immediately.
Please cooperate, whether you make the mess or not it needs to be cleaned.
Breaking
of instruments, glassware, or chemical containers should be cleaned up properly and reported to the laboratory manager.
After an experiment you should
properly clean all the areas/equipment
you worked on.
Ie
ice buckets must be emptied, dried and put back in the designated cabinet.
Keep lab bench tidy and free of clutter.
Wash hands
when you leave the laboratory.Slide5
Organization and Courtesy
New technique: Each lab member has their designated color of tape.
All bottles must be labeled with the bottle’s contents, your name, and date it was made.All boxes must be labeled with your color tape, your name, date, and box ID. Records should be taken of all boxes and a copy should be given to the laboratory manager.Freezer/Fridge.Your
Initials.Box Number.3letter descriptionExample: A1.CG.4.T4A Only put your things in your assigned areas
ie
freezers, fridges.
Each lab member has their own tips,
pipettors
, tubes, and place to keep open products
ie
serological
pipets
, 50ml tubes.
DO NOT
put open products back with the stocks on the sterile shelf.
Never
use someone else’s media, stock solutions,
labware
(
pipettemen
, tips, tubes, etc.) or special chemicals without their consent. If you borrow something (
w
/their consent) please put it back when finished.Slide6
Orders
Orders are made Monday and Wednesday. Before Noon.
Please plan ahead.If you use the last of something write the order on the board. Keep orders from other vendors together to save on shipping.Order the smallest quantity you need for your whole experiment. If you are going to use it, and it is cheaper then feel free to order the larger concentration/volume.All lab members must participate in opening and putting away orders. All packing slips must be put on the lab manager’s designated shelf with your initials and date it was received.Orders should be put in the designated areas (4°,-20°), so they are easier to find.Slide7
Orders Continued
What I need to be efficient:
Product Name, Catalogue Number, Vendor, Price, Initials, PriorityCheck the catalogue number on the vendor’s website, do not just put the number on the item.Vendor means person who sells the product, not necessarily person who makes the product.Unless the product is on Fishersci.com provide the price of the item.Please DO NOT put any orders on the whiteboard without a catalogue number and your initials.
A list has been put on the whiteboard with the details of commonly used items.Slide8
Laboratory Equipment
If you have questions about any instruments (etc.), please ask before you use the instrument.
Scales are shared equipment and should ALWAYS be cleaned and shut off after use
.If you use an instrument, make sure the instrument and the area around it is clean when you are done.
All equipment should be
turned off
when you are finished or at the end of the day. Don’t assume someone else will turn it off, we want to keep our instruments in good working condition.
If something explodes or spills clean it thoroughly.
Always discard used tubes, bacteria plates, etc. left in incubators/freezers.Slide9
Biosafety
Hoods
Wear double gloves when working with infectious agents.All hoods must be cleaned thoroughly once a month.Nothing should be kept in the hoods when finished and when in use clutter should be at a minimum.Discard your biological waste after use ie
. Pipet tips, loops, and used medium.Hoods should be cleaned with
70% ethanol before and after use.
N.
meningitidis
users should wear a dust mask and glasses when handling bacteria.Slide10
Waste Disposal and Dishes
Large red waste boxes are for
biohazardous materials/chemically exposed materials. Please do not throw non-contaminated materials in this waste box.Gray waste bins are for paper, Styrofoam, weigh boats, paper towels, fed ex bubble envelopes etc.Hazardous chemicals should be put into an empty plastic bottle with a hazardous sticker and placed in the satellite accumulation area.
Liquid and solid waste containing Ethidium Bromide goes into its proper container.
All dirty glassware should be put onto the dirty dish cart.
Labels should be removed
All contents of container should be removed.
Agar should not be allowed to cool completely, water should be put into the bottle and all agar removed before putting on dish cart.Slide11
Laboratory Coats
Please hang up your laboratory coats.
Cell culture/Clean reagents—Purple, Pink Coats.Bacteria—Blue Coats.Label your Coats. Do not hoard coats, keep only two and throw the rest away.Slide12
Laboratory Duties
Each person will be assigned a lab duty each month. This is your responsibility and it is up to you to see that it is done. Note: some duties are required 2-3x every month.
We will have a card for each duty, at the end of each month at lab meeting we will exchange the duty for another.List of Duties:Autoclaving pipet tips. Putting away empty boxes for later use 1-2x per month (1 person).Dishwashing. 2xper month (1 person).Hood cleaning: 1xper month x5hoods
(5 people).Incubator cleaning: 1xper month x2(cell/bacteria) (2 people)
Ethidium
Bromide station cleaning: 1xper month (1 person)
When using large quantities of
pipet
tips, tubes or glassware please prepare your own tips and wash your own glassware.
IncentiveSlide13
Final Notes
The laboratory is a working environment in which we all must cooperate by keeping our
workstation and shared equipment/stations clean.The custodian does not clean after us and we do not have a maid, so please keep things clean and neat.Gloves are not expensive, change them as often as you like.Overall, always use common courtesy when working in the laboratory.
Let’s try to keep Eve out of our laboratory.