Daheeya ALEnazi Principle Triglycerides H 2 O Lipase mono diglycerides Fatty acids Serum is incubated at 37C with an olive oil substrate buffered at pH 90 triglycerides will hydrolysed in presence of the olive oil by pancreatic lipase this cause ID: 631319
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Slide1
LAB 8LIPASE
Quantitative turbid-metric determination of Pancreatic Lipase in serum or plasma
Daheeya
ALEnaziSlide2
Principle
Triglycerides + H
2
O
Lipase mono + di-glycerides + Fatty acids Serum is incubated at 37C with an olive oil substrate buffered at pH 9.0. triglycerides will hydrolysed in presence of the olive oil by pancreatic lipase this cause :a decrease in the turbidity of the reaction mixture.*The decrease in absorbance is measured and reflects the activity of lipase in the sample.Slide3
Clinical Diagnosis
Elevation of LPS is used for:Diagnosis of pancreatic disease such as acute and chronic pancreatitis and obstruction of the pancreatic duct.Slide4
Procedure
blank
Test
WR-ml
33
Incubate at 37 C for 5 min.
Sample-µl
--
100
Read Abs. at 400nm against D.W as AO for Blank and testIncubate at 37 C for 5 min.Read Abs A1 for blank and test.Calculate A .
blank
Test
WR-ml
3
3
Incubate at 37 C for 5 min.
Sample-µl
--
100Slide5
Calculation and normal range
Test (A0 – A1) – blank (A0 – A1) x 3000 = lipase activity in U/L
blank (A0)
Reference values:
ADULTS: 10 – 150 U/L