PDF-Why Live Imaging?1.Confocal and Multi-photon Fluorescence Microscopy 2

httpwwwglassbottomdishescom Medium aging where fluorescent molecules are Information regarding protein mobilitymeasuring the rate of fluorescenc Why Does Fluorescence Recover PrebleachPartia. Why live cell imaging?. Live cell analysis provides direct spatial and temporal information. Planning your experiment . The markers/. fluorophores. The cell’s environment. Practical aspects of the experiment: the microscope. BIF Microscopy Workshop. March 25. th. , 2015. Director: Professor Thomas V. O’Halloran. Managing Director: Keith MacRenaris, . Ph.D. History and Mission of CLP and QBIC. “The . Chemistry of Life Processes Institute acts as an umbrella for a variety of centers, facilitates collaborations and helps bridge different cultures. By lowering the barriers to scientific discovery, the Institute hopes, for example, to design new drugs for the treatment of cancer and neurodegenerative diseases as well as develop improved techniques for diagnosing diseases earlier.” . A Statistical Study and . Potential Software Tools For Practitioners . Carol . Gambino. , Patrick McLaughlin, Loretta . Kuo. , . Peter . Diaczuk. , Gerard . Petillo. , . Frani. . Kammerman. , Lauren . microlens. array. Antony Orth and Kenneth . Crozier. 8 . May. CLEO . 2012. Microscopy with lens arrays. What is high . thoughput. microscopy?. Experimental setup – . confocal. system. Lens array characteristics, resolution. Microlens. Array. 18 October, . FiO. 2011. Antony Orth and Kenneth . Crozier. High Throughput Microscopy. 1. http://www.olympus.co.uk/microscopy/22_scan_R.htm#. High throughput fluorescence imaging by scanning sample under . Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. . What is a . fluorescence. ?. Wiki. : emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength.. The name coined by George Gabriel Stokes in 1852 “. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. di . microscopia. ad . illuminazione. . strutturata. per studio di . fenomeni. . veloci. Relazione. . di . dottorato. . A.A.:2012 . Ciclo. XXVII. Dottorando. : Paolo . Pozzi. Tutore. : Giuseppe Chirico. Lecture 18:. High speed microscopy, Part 2. High speed microscopy, Part . 2: Spatial . light modulator microscope and other 3D sensors. Making laser scanning confocal microscopes faster. Resonant scanner confocal. lecture. 16. November 2011. Fourier. plane. Point . object. Image. f. f. f. f. a. a. ’. Magnification. : m=1. Angles. : sin(. a. ’)=sin(. a) .  . a. ’=. a. Magnification and resolution: the Abbe limit. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. .