PPT-Confocal Microscopy Confocal Microscopy

Outline Introduction Optical Microscopy Types of Optical Microscopes Confocal Microscopy Laser Scanning Confocal Microscopy Examples Optical Microscopy Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. Weeks Emory University Atlanta Georgia USA INTRODUCTION A confocal microscope creates sharp images of a speci men that would otherwise appear blurred when viewed with a conventional microscope This is achieved by excluding most of the light from the Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. BIF Microscopy Workshop. March 25. th. , 2015. Director: Professor Thomas V. O’Halloran. Managing Director: Keith MacRenaris, . Ph.D. History and Mission of CLP and QBIC. “The . Chemistry of Life Processes Institute acts as an umbrella for a variety of centers, facilitates collaborations and helps bridge different cultures. By lowering the barriers to scientific discovery, the Institute hopes, for example, to design new drugs for the treatment of cancer and neurodegenerative diseases as well as develop improved techniques for diagnosing diseases earlier.” . A Statistical Study and . Potential Software Tools For Practitioners . Carol . Gambino. , Patrick McLaughlin, Loretta . Kuo. , . Peter . Diaczuk. , Gerard . Petillo. , . Frani. . Kammerman. , Lauren . microlens. array. Antony Orth and Kenneth . Crozier. 8 . May. CLEO . 2012. Microscopy with lens arrays. What is high . thoughput. microscopy?. Experimental setup – . confocal. system. Lens array characteristics, resolution.             Zeiss LSM 510 META - Guided TourConfocal microscopyVisualisation of biological structures in 3D Carl Zeiss - Training Application and Support Center Zeiss LSM 510 META - Guided Tour Confocal Principl lecture. 16. November 2011. Fourier. plane. Point . object. Image. f. f. f. f. a. a. ’. Magnification. : m=1. Angles. : sin(. a. ’)=sin(. a) .  . a. ’=. a. Magnification and resolution: the Abbe limit. Products:. ► Centaur . ► Centaur HR. ► Snotra. ► Certus Optic. ► Certus Standard. ► Certus Light. ► Ratis. Where to use:. ► Biology. ► Chemistry. ► Physics. ► Interdisciplinary research: . II. MENA3100,OBK, . 29.01.15. We don’t read all. You don’ have to read all. 1.3 Specimen Preparation. Read it. 1.4.1 Bright-Field and Dark-Field. Definetly. 1.4.2 Phase-Contrast. Cursori. 1.4.3 Polarized-Light. Lecture 16:. Super-resolution microscopy: Part . 2. Lecture 16: Super-resolution microscopy and TIRFM. Single molecule imaging. Total internal reflection fluorescence microscopy (TIRFM). Super-resolution techniques . Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . Foucault C, Brouqui P, Raoult D. Bartonella quintana Characteristics and Clinical Management. Emerg Infect Dis. 2006;12(2):217-223. https://doi.org/10.3201/eid1202.050874.