PPT-Confocal Microscopy Confocal Microscopy

Outline Introduction Optical Microscopy Types of Optical Microscopes Confocal Microscopy Laser Scanning Confocal Microscopy Examples Optical Microscopy Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. Confocal Application Letter March 1997 Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. Yasuhiro Mukaigawa (Osaka University). Seiichi Tagawa (Osaka University). Jaewon Kim (MIT Media Lab). Ramesh Raskar (MIT Media Lab). Yasuyuki Matsushita (Microsoft Research Asia). Yasushi Yagi (Osaka University). Confocal Application Notes Vol. 5 May 2007 Leica Microsystems Inc. Toll Free 866-830-0735, Option 3 Application and Technical Support Fax (610)-321-0425 410 Eagleview Blvd, Ste. 107 www.conf Confocal Application Notes Vol. 4 October 2006 Leica Microsystems Inc. Telephone (610)-321-0460 410 Eagleview Blvd, Ste. 107 Fax (610)-321-0425 www.confocal-microscopy.com Exton, PA 19341 2 II. MENA3100,OBK, . 29.01.15. We don’t read all. You don’ have to read all. 1.3 Specimen Preparation. Read it. 1.4.1 Bright-Field and Dark-Field. Definetly. 1.4.2 Phase-Contrast. Cursori. 1.4.3 Polarized-Light. Fire Protection Laboratory Methods Day. June 25, 2014. Paul M. Anderson. Graduate Research Assistant. University of Maryland. Department of Fire Protection Engineering. Transmission Electron Microscopy. Lecture 16:. Super-resolution microscopy: Part . 2. Lecture 16: Super-resolution microscopy and TIRFM. Single molecule imaging. Total internal reflection fluorescence microscopy (TIRFM). Super-resolution techniques . Jenny . Malmstrom. AFM invented by Binning and co-workers in 1986. . Belongs to the Scanning Probe Microscopy family. Binning et al., Physics Review Letters 1986. AFM invented by Binning and co-workers in 1986. . Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. Microscopy is the technical field using microscopes to view samples and objects that can not be seen without unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron and scanning probe microscopy.. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co Amina Mustafa. Department of Molecular Biology. Virtual University of Pakistan. Invented by :. Antonie. van Leeuwenhoek. (Father of Microbiology). Microscopy. USES:. Diagnostics. Identification. Histology – Tissue analysis. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. .