PPT-FLUORESCENCE MICROSCOPY PRESENTED BY

KAARTHIK J INTRODUCTION A fluorescence microscope is a optical microscope that uses fluorescence and phosphorescence instead of or I addition to reflection and absorption to study properties of organic or inorganic substances. AIT. Exercise . 2.1. Atomic. Molecular. Absorption. Emission. flame AAS. UV/VIS. IR. flame emission. ICP. ??. Fluorescence. Interaction with radiation. absorption to exc. state via heat, electricity, radiation. Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. . What is a . fluorescence. ?. Wiki. : emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength.. The name coined by George Gabriel Stokes in 1852 “. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. lecture. 16. November 2011. Fourier. plane. Point . object. Image. f. f. f. f. a. a. ’. Magnification. : m=1. Angles. : sin(. a. ’)=sin(. a) .  . a. ’=. a. Magnification and resolution: the Abbe limit. Lecture 16:. Super-resolution microscopy: Part . 2. Lecture 16: Super-resolution microscopy and TIRFM. Single molecule imaging. Total internal reflection fluorescence microscopy (TIRFM). Super-resolution techniques . and Kavantzas N., "Computer vision algorithms in DNA ploidy image analysis", Imaging, Manipulation and Analysis of Biomolecules, Cells and Tissues IV, Proc. SPIE, 6088:60880O (2006) and Loukas S., "A Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co Lecture 06: Fluorescence Microscopy. Andres Collazo, Director Biological Imaging Facility. Ke Ding, Graduate Student, TA. Wan-Rong (Sandy) Wong, Graduate Student, TA. Lecture 6: Fluorescence Microscopy. Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. Marina . Alterman. , . Yoav. . Schechner. Aryeh. Weiss. Technion. , Israel. Bar-. Ilan. , Israel. 2. Natural Linear Mixing. Raskar. et al.. 2006.. ImageJ. image sample. collection.. c. c. i. i. are held together at their centromeres binds specific proteins, which in turn make up a disk-like structure called the kinetochore. The kinetochore is an attachment site for spindle fibers, which pla Begum. Assistant Professor. Dept. of Microbiology. Jamal Mohamed College. Introduction. Microscopy is the technology of making very small things visible to naked eyes.. The science of investigating small objects using such an instrument is called microscopy.. Dr.. A. K. Dhingra. Indocyanine. . G. reen Dye - ICG. w. as . developed for near- infrared (NIR) photography by . Kodak Research . Labs. . in 1955 and was introduced in clinical practice . in . 1956 .