PPT-Bi/BE 177: Principles of Modern Microscopy

Lecture 06 Fluorescence Microscopy Andres Collazo Director Biological Imaging Facility Ke Ding Graduate Student TA WanRong Sandy Wong Graduate Student TA Lecture 6 Fluorescence Microscopy. Lecture . 09: . Polarization and DIC. Lecture 9: Polarization and DIC. Review Contrast and Phase Contrast. Polarization. Birefringence. Nomarski. . (Differential . Interference Contrast). Resolution. Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Microlens. Array. 18 October, . FiO. 2011. Antony Orth and Kenneth . Crozier. High Throughput Microscopy. 1. http://www.olympus.co.uk/microscopy/22_scan_R.htm#. High throughput fluorescence imaging by scanning sample under . Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. Lecture 06: . Fluorescence Microscopy. Lecture . 6: Fluorescence . Microscopy. Detectors for Microscopy, Part 2. CMOS, PMT and APD. Phenomenon . of Fluorescence. Energy . Diagram. Rates . of excitation, emission, ISC. Lecture 18:. High speed microscopy, Part 2. High speed microscopy, Part . 2: Spatial . light modulator microscope and other 3D sensors. Making laser scanning confocal microscopes faster. Resonant scanner confocal. Lecture . 08: . Contrast and Resolution. Lecture 8: Contrast and Resolution. Bright-field. Tinctorial. dyes: the first contrast. Review . of Kohler Illumination. Tradeoffs . in Contrast/Resolution. Dark . II. MENA3100,OBK, . 29.01.15. We don’t read all. You don’ have to read all. 1.3 Specimen Preparation. Read it. 1.4.1 Bright-Field and Dark-Field. Definetly. 1.4.2 Phase-Contrast. Cursori. 1.4.3 Polarized-Light. Microscopy is the technical field using microscopes to view samples and objects that can not be seen without unaided eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron and scanning probe microscopy.. Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co 1Many different types of light microscopesare available each with their own strengths and limitationsA main tenant of good microscopy is to select the right tool microscope for the job at handPlease c KAARTHIK J. INTRODUCTION. A fluorescence microscope is a optical microscope that uses fluorescence and phosphorescence instead of , or I addition to reflection and absorption to study properties of organic or inorganic substances.. AIM : T THEORY: Microscope is the most commonly used piece of apparatus in the laboratory. It produces greatly enlarged images of minute objects. LIGHT MICROSCOPE A Light Microscope can be simple or