PPT-Figure Figure. . . . Dendrogram of NotI-digested pulsed-field gel electrophoresis (PFGE)

Chowdhury G Pazhani GP Dutta D Guin S Dutta S Ghosh S et al Vibrio fluvialis in Patients with Diarrhea Kolkata India Emerg Infect Dis 2012181118681871 httpsdoiorg103201eid1811120520. By Andrew Gioe and Ben Berger. Electrophoretic Experiments. Free Electrophoresis or Moving Boundary Electrophoresis. Done in solution with no support Medium. No longer widely used due to problems resulting from the formation of convection currents in the solution from heating.. Electrophoresis. • . Separation technique based on the movement of . analyte. through a conductive medium in response to an applied electrical field.. • . The medium is usually a buffered aqueous. INTRODUCTION. Electrophoresis is a migration of a charged particle in a medium under the influence of an applied electric field.. The usual purposes of carrying out . electrophoretic. experiments are :. Lab.8. https://www.youtube.com/watch?v. =. 8RBs0Ghg_48. Gel Electrophoresis. Gel Electrophoresis. Gel electrophoresis apparatus.  An . agarose. gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear.. how does it work?. Technique used to separate samples of DNA, RNA, and protein according to charge and/or size. Smaller molecules move farther and faster through the . agarose. gel. Opposite charges each other. Tasnuva. . Jhileek. Dr. Francine . Norflus. Biotechnology. What is Gel Electrophoresis?. A procedure that separates molecules . based on size and charge.. . Uses an electric field. Molecules move through . 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. GEL ELECTROPHORESIS. Sep. Of proteins Based on molecular wt only. Based on mol. Wt. & . Vincent C, Boerlin P, Daignault D, Dozois CM, Dutil L, Galanakis C, et al. Food Reservoir for Escherichia coli Causing Urinary Tract Infections. Emerg Infect Dis. 2010;16(1):88-95. https://doi.org/10.3201/eid1601.091118. Cady A, Plainvert C, Donnio P, Loury P, Huguenet D, Briand A, et al. Clonal Spread of Streptococcus pyogenes emm44 among Homeless Persons, Rennes, France. Emerg Infect Dis. 2011;17(2):315-317. https://doi.org/10.3201/eid1702.101022. Vandenesch F, Naimi T, Enright MC, Lina G, Nimmo GR, Heffernan H, et al. Community-Acquired Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Genes: Worldwide Emergence. Emerg Infect Dis. 2003;9(8):978-984. https://doi.org/10.3201/eid0908.030089. Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . Wedel SD, Bender JB, Leano FT, Boxrud DJ, Hedberg C, Smith KE. Antimicrobial-drug Susceptibility of Human and Animal Salmonella Typhimurium, Minnesota, 1997–2003. Emerg Infect Dis. 2005;11(12):1899-1906. https://doi.org/10.3201/eid1112.050158. Peters PJ, Brooks JT, McAllister SK, Limbago B, Lowery H, Fosheim G, et al. Methicillin-Resistant Staphylococcus aureus Colonization of the Groin and Risk for Clinical Infection among HIV-infected Adults. Emerg Infect Dis. 2013;19(4):623-629. https://doi.org/10.3201/eid1904.121353. Cerquetti M, degli Atti ML, Cardines R, Salmaso S, Renna G, Mastrantonio P. Invasive Type e Haemophilus influenzae Disease in Italy. Emerg Infect Dis. 2003;9(2):258-261. https://doi.org/10.3201/eid0902.020142.