PPT-Figure Figure. Pulsed-field gel electrophoresis (PFGE) patterns of SmaI-restricted

Cady A Plainvert C Donnio P Loury P Huguenet D Briand A et al Clonal Spread of Streptococcus pyogenes emm44 among Homeless Persons Rennes France Emerg Infect Dis 2011172315317 httpsdoiorg103201eid1702101022. Chelsea Aitken. Peter Aspinall. Zonal Electrophoresis. Most common form of electrophoresis in biological studies. Uses a support system, most commonly gel to separate proteins by their properties. We will cover methods to separate by:. October 15. th. – October 19. th. , 2012. Gel Electrophoresis. The process by which electricity is used to separate charged molecules (. DNA fragments, RNA, and proteins. ) based on there size, shape, and charge. . Lab.8. https://www.youtube.com/watch?v. =. 8RBs0Ghg_48. Gel Electrophoresis. Gel Electrophoresis. Gel electrophoresis apparatus.  An . agarose. gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear.. 1. Migration of charged particles on supporting media. Migration of charged particles in solution. No supporting media. 2. GEL ELECTROPHORESIS. Sep. Of proteins Based on molecular wt only. Based on mol. Wt. & . To prepare 4% NuSieve agarose gel (14 x 10 x 0.5 cm) take the following in a 500 ml Agarose: 1.6 g NuSieve agarose: 1.6 g 1 X AGB buffer 80 ml Fill the electrophoresis tank with 1 x TBE buffer. Plac Jhung MA, Thompson AD, Killgore GE, Zukowski WE, Songer G, Warny M, et al. Toxinotype V Clostridium difficile in Humans and Food Animals. Emerg Infect Dis. 2008;14(7):1039-1045. https://doi.org/10.3201/eid1407.071641. Hageman JC, Pegues DA, Jepson C, Bell RL, Guinan M, Ward KW, et al. Vancomycin-Intermediate Staphylococcus aureus in a Home Health-Care Patient. Emerg Infect Dis. 2001;7(6):1023-1025. https://doi.org/10.3201/eid0706.010618. Doublet B, Lailler R, Meunier D, Brisabois A, Boyd D, Mulvey MR, et al. Variant Salmonella Genomic Island 1 Antibiotic Resistance Gene Cluster in Salmonella enterica Serovar Albany. Emerg Infect Dis. 2003;9(5):585-591. https://doi.org/10.3201/eid0905.020609. Principle. Factors affecting the distance of movement. Application. Polyacrylamide Gel Electrophoresis (PAGE). Hemoglobin Electrophoresis. Objectives. Electrophoresis is a process distinguishing and isolating different compounds from each other. . Wedel SD, Bender JB, Leano FT, Boxrud DJ, Hedberg C, Smith KE. Antimicrobial-drug Susceptibility of Human and Animal Salmonella Typhimurium, Minnesota, 1997–2003. Emerg Infect Dis. 2005;11(12):1899-1906. https://doi.org/10.3201/eid1112.050158. By Angel Luis Vázquez Maldonado. November 8. th. , 2018. Gel Electrophoresis and its Purpose. 2. Electrophoresis is derived from Greek. Electro . – refers to the electrical current that adds energy to the electrons of the molecule’s atoms. Tassios PT, Chadjichristodoulou C, Lambiri M, Kansouzidou-Kanakoudi A, Sarandopoulou Z, Kourea-Kremastinou J, et al. Molecular Typing of Multidrug-Resistant Salmonella Blockley Outbreak Isolates from Greece. Emerg Infect Dis. 2000;6(1):60-64. https://doi.org/10.3201/eid0601.000111. Peters PJ, Brooks JT, McAllister SK, Limbago B, Lowery H, Fosheim G, et al. Methicillin-Resistant Staphylococcus aureus Colonization of the Groin and Risk for Clinical Infection among HIV-infected Adults. Emerg Infect Dis. 2013;19(4):623-629. https://doi.org/10.3201/eid1904.121353. Cerquetti M, degli Atti ML, Cardines R, Salmaso S, Renna G, Mastrantonio P. Invasive Type e Haemophilus influenzae Disease in Italy. Emerg Infect Dis. 2003;9(2):258-261. https://doi.org/10.3201/eid0902.020142.