PPT-Protein Quantitation II: Multiple Reaction Monitoring

Kelly Ruggles kellyfenyolaborg New York University Lecture Outline Protein quantitation using MSMS Basics of targeted proteomics Motivating example AKT and breast cancer Lecture Outline . Joshua J. Coon. U. Wisconsin-Madison. Isobaric Tagging. Ross,PL. . et al. . MCP. . 2004. iTRAQ. Structure. Available Reagents. Phosphorylation. site on LIN28. Contemporary proteomics. Introduction of internal standard. Case Study . Challenges . and Successes. www.envirotechsystems.com. www.advancedsensors.co.uk. info@envirotechsystems.com. sales@envirotechsystems.com. 17327 Norwell Drive. Covington, . LA . 70435. Phone: 985-809-6480. (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors in which they take place.. Lecture. 12. Today’s. . lecture. Multiple Reactions. Selectivety. Biuret. &Lowery Reactions. CLS 331. Exp.no.5. Introduction. There are many methods for the determination of . the amount of . protein in a natural product.. A particular method is chosen in accordance with materials to be . Synapse-Associated Protein 97. Wu et al, 2002. EMBO J 19:5740-5751. Domain Analysis and Protein Families. Introduction. What are protein families?. Motifs and Profiles. The modular architecture of proteins. UNIT 1. Question 1. . When . selecting quality fruit it should be:. a. plump. b. free . from blemishes and bruises. c. heavy . for size. d. all . of the above. Question 1. . When . selecting quality fruit it should be:. Multiple Use MonitoringBest available scientific information BASI the responsible official shall document in the decision document how BASI was used to inform the plan monitoring program This document Vi Dao, MD, FRCPC. vdao@cancercare.mb.ca. Presenter Disclosure. Faculty: Vi Dao. Relationships with commercial interests: none. Mitigating Potential Bias. Not Applicable. Learning Objectives. Distinguish MGUS from multiple myeloma. Enzymatic action. Classification of enzymes. Enzyme class. Reaction type. Description. EC 1. Oxidoreductases. Catalyze oxidation reduction reaction, e.g., oxidases, dehydrogenases, . oxygenases. , etc.. PROTEOMICS. (. Mass spectrometry in Biochemistry). LC-MS. 2. Sample inlet systems for ESI. Mass analyzers. 3. S/N = 1. 1.0 ml/min. 4.6 mm i.d.. S/N= 3800. 75 . . m i.d.. S. ignal. to. Noise ratio. quantitation. . -. For most K residues our histone assay, we monitor the possible occurrence of difference modifications (. m. e1. , . m. e2. , . m. e3. ,. Ac. ) and . unmodified. peptide. . -Different forms of the same peptide, apart of their mass differences, can have different retention times (Important for . Mechanism of folding and . misfolding. GroEL. – biological machine (chaperones folding). Molecular motors: Polymer physics and Myosin V motility. Many Facets of Folding . Structure Prediction. Protein & Enzyme Design. . Mikhail Belov, Satendra Prasad, David Prior, William Danielson, Karl Weitz, Vladislav Petyuk, Yehia Ibrahim and Richard Smith. Pacific Northwest National Laboratory, Richland, WA. Introduction. Overview. Acids. By . Professor . Dr. Jamal Ahmed Abdel -Barry. . Rare Amino Acids. In addition to 20 standard amino acids there are several amino acids that can be isolated from the hydrolysis of special type of protein, all are derivatives of some standard amino acids..