PDF-DIGE versus Coomassie Staining

and Silver StainingKendrick Labs Inc Madison WIwwwkendricklabscom1OverviewThe following slides present a comparison of 2D TwoDimensional Difference Gel Electrophoresis DIGE utilizing Cyanine Dyes Cy. Ettan DIGE system includes CyDye DIGE fluor dyes Typhoon 9400 Variable Mode Imager and DeCyder Differential Analysis Software The three currently available CyDye DIGE fluors used in Ettan DIGE system are each spectrally resolvable and are size and c The former represents a centralized approach while the latter represents a decentralized approach The centralized IT department suits some organizations better than others However it is becoming increasingly obvious that organizations may often cons You see, many Myth versus Reality: Choosing Gender and Age people see the Golden Retriever a great family dog. While this is true, don'tlose sight of what the Golden Retriever was originally bred for HAEMATOXYLIN & EOSIN. . Supervised by : . Dr.Sherifa. . Prepared by: Reem Aldossari. Lama . Alabdi. H&E staining:. The best known and most widely used staining procedure, . hematoxylin. -eosin staining (H&E), uses . (BD Biosciences Protocol) Materials: Staining medium (SM) [1X HBSS; 2% (v/v) calf serum; 10mM NaN 3 10 mM HEPES, pH 7.2] Sterile filtered calf serum (CS) [0.45m TC sterile filtered] Gey’s solu Lab #4 . Medgar Evers College. Bio 261 Microbiology. Prof. Santos. Aim #1 exercise 14. Capsular staining. Capsule- extracellular layer composed of polysaccharides found in some bacteria that plays a role in immunity and adhesion.. Staining. Coloring organism with a dye. Microorganisms must be . FIXED. to microscopic slide first. Kills microbe. Attaches organism to the slide. Preserves various parts with minimal distortion. Steps to fix:. A.L. Noor Ameer . The reasons of staining. 1- To study their shapes . 2- . To differentiate the species of bacteria by using differential . stain.. 3- . To study the internal components of the bacterial . Ettan Important user informationAll users must read this entire user manual to fully understand the safe use of Ettan DIGE System. For safe use of Ettan DIGE System related products described in this - D DIGE Huadong Liu Product Specialist Huadong.liu@ge.com GE Healthcare Life Sciences China 2 / GE DIGE / 3/22/2012 Agenda 1. 2 - D DIGE concepts and benefits 2. Biomarkers in colorectal cancer 3. The DIGE System comprises a defined procedure for 2D that uses an internal standard to generate the most accurate resultsDIGE system is based on the technique of twodimensional difference gelelectroph Faculty: Dr. Rakesh Sharda OBJECTIVES OF STAINING Improves visibiltiy by greater contrast between the organism and the background, differentiate various morphological types (by shape, size, arrangeme This. procedure is called . so,when. certain bacteria or some of their . structures could not be stained or seen or being differential with other bacteria cells when using the ordinary . staining,such. Fixatives used in histopathology. 2. Staining. Differential staining. Gram’s staining. Vital staining. Trypan. blue- dead cells take up the stain . i.e. stain positively and live cells . Exclude the stain or stain negatively.