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Creating a heterozygous male Creating a heterozygous male

Creating a heterozygous male - PowerPoint Presentation

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Uploaded On 2016-03-12

Creating a heterozygous male - PPT Presentation

Hermaphrodite Parent genotype Male Parent genotype X Male gametes Female gametes Progeny genotype Which progeny are you continuing with Why Progeny phenotypes and ratios Hermaphrodite ID: 252591

progeny genotype male gametes genotype progeny gametes male hermaphrodite parent unc cross phenotypes ratios female linkage mapping selfing linked

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Presentation Transcript

Slide1

Creating a heterozygous male

Hermaphrodite Parent genotype

Male Parent genotype

X

Male gametes

Female gametes

Progeny genotype

Which progeny are you continuing with? Why?

Progeny phenotypes

and ratiosSlide2

Hermaphrodite

Parent genotype

Male Parent genotype

X

Male gametes

Female gametes

F1 Progeny genotype

and ratios

F1 Progeny phenotypes

Linkage 1: Cross with Unlinked

unc

+/+ ;

unc/uncSlide3

Linkage 2: Selfing of hermaphrodite from unlinked cross

hermaphrodite

X

self cross

gametes

Progeny genotype

Progeny phenotypes

and ratiosSlide4

Hermaphrodite

Parent genotype

Male Parent genotype

X

Male gametes

Female gametes

Progeny genotype

Progeny phenotypes

and ratios

Linkage 1: Crossing with Linked

unc

Which progeny are you continuing with? Why?

+

unc/

+

uncSlide5

Linkage 2: Selfing of hermaphrodite from linked cross

hermaphrodite

X

self cross

gametes

Progeny genotype

Progeny phenotypes

and ratiosSlide6

Mapping 1: Making the double mutant

hermaphrodite

X

self cross

gametes

Progeny genotype

Progeny phenotypes

and ratios

What if the genotype of the parent was

+

unc

/+

unc

?Slide7

Mapping 2: Making heterozygotes for mapping

Hermaphrodite Parent genotype

Male Parent genotype

X

Male gametes

Female gametes

Progeny genotype

Progeny phenotypes

and ratios

Why might you see

Dpy

Unc

progeny?Slide8

Mapping 3: Test Cross

Hermaphrodite Parent genotype

Male Parent genotype

X

Male gametes

Female gametes

Progeny genotype

Progeny phenotypes

and ratios

Parental

RecombinantSlide9

Symbols to Know for Crosses

a//a – a is the allele and // represents the chromatids – there are two for a diploid organism – sometimes written as /a b//a b – two different genes on the same chromosomea//a ; b//b – two different genes on different chromosomesX – symbolizes mating between two different individualsX - symbolizes a self cross – when the hermaphrodite worms fertilize their own eggs