PPT-Julia Paxson DVM DACVIM Analysis of Gene Expression

Overview Why gene expression analysis Quantification of mRNA transcript abundance High specificity high throughput Requires sequence knowledge Considerations Experimental question Species limitations . Lukasik DVM DACVA W Sean Penn DVM DABVP CanineFeline Brett Sargent DVM DABVP CanineFeline Kim Spelts CVT VTS CCRP Anesthesia ABSTRACT Safe and effective anesthesia of dogs and cats rely on preanesthetic patient assessment and preparation Patients sh Lukasik DVM DACVA W Sean Penn DVM DABVP CanineFeline Brett Sargent DVM DABVP CanineFeline Kim Spelts CVT VTS CCRP Anesthesia ABSTRACT Safe and effective anesthesia of dogs and cats rely on preanesthetic patient assessment and preparation Patients sh Inducible gene expression. kinetics of . β-galactosidase. enzyme induction. Add inducer. start transcription = mRNA accumulation. mRNA translation = protein accumulation. Remove inducer. Stop. transcription (. The problem to be solved (an example). Hauf, J., Zimmermann, F.K., M. ü. ller, S., 2000. Simultaneous genomic over expression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Ezyme. Microbiol. Technol.. Bing Zhang. Department of Biomedical Informatics. Vanderbilt University. bing.zhang@vanderbilt.edu. Overall workflow of gene expression studies. Microarray. Biological question. Experimental . design. What does the operon model attempt to explain?. the coordinated control of gene expression . in bacteria. bacterial resistance to antibiotics. how genes move between homologous regions of DNA. the mechanism of viral attachment to a host cell. MacCaig. We are learning to:. We are learning to: annotate the text and identify the use of poetic techniques. . Two more to go! . . Context of the poem. Norman MacCaig's Aunt Julia lived on . Scalpay. University of Notre Dame. Kristin Hager, Associate Teaching Professor, Biological Sciences. Joseph O’Tousa, Professor, Biological Sciences. WH Freeman. Marc Mazzoni, Senior Editor, Life Sciences. Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College. Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. trolchecks,andscheduled,whichdoestheactualmeasurementscheduling,
execution,andresultpackaging.Asindicatedabove,securityisabasicpartoftheNIMIar-chitecture.Authenticationandencryptionofallcommunicationb Drosophila. Embryos using . lacZ. Transgenes. June 18. th. ABLE 2014. University of Oregon, Eugene. Cathy Silver Key. Julie Gates. Jessica Sawyer. Kirsten . Guss. Acknowledgements. Funding from . Roberta . Assessment of murmurs in variousASYMPTOMATICpatient populations: P a tient Possible differentials Recommended diagnostics Puppy or kitten with a soft systolic murmur Innocent flow murmur These mur Definitions Alopecia = loss of hairHypotrichosis = presence of less than the normal amount of hairMost commonly used in reference to congenital disorders Causes of AlopeciaFailure of hair growthEndocr What is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. RNAseq. data. DE analysis based on . RNAseq. data.