PPT-V3 – differential gene expression analysis

What is measured by microarrays Microarray normalization Differential gene expression DE analysis based on microarray data Detection of outliers RNAseq data DE analysis based on RNAseq data. limma. package. 20 March 2012. Functional Genomics. Linear regression. Fit a straight line through a set of points such that the distance from the points to the line is minimized. The slope of the line is adjusted to minimize the . Ju. . Han Kim . Division of Biomedical Informatics, Seoul National University College of Medicine, Seoul, Korea, . Presenter: Zhen Gao . 2. Outline. Review of major computational . approaches to facilitate biological interpretation of . The problem to be solved (an example). Hauf, J., Zimmermann, F.K., M. ü. ller, S., 2000. Simultaneous genomic over expression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Ezyme. Microbiol. Technol.. FREQUENCY 10k 69 72 75 78 81 84 87 90 GAIN TA01b 100k 1M 10M 100M 1G 60 63 66 Pseudo-Differential Differential)/95dB (Pseudo-Differential)Programmable Compression VoltagesOnboard Reference Reference Alisha Holloway, PhD. Gladstone Bioinformatics Core Director. What is RNA-seq?. Use of high-throughput sequencing technologies to assess the RNA content of a sample.. Why do an RNA-seq experiment?. Detect . University of Notre Dame. Kristin Hager, Associate Teaching Professor, Biological Sciences. Joseph O’Tousa, Professor, Biological Sciences. WH Freeman. Marc Mazzoni, Senior Editor, Life Sciences. Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College. TUMOR SUPRESSOR PROTEINS. ATP. ENZYMES THAT CONTROL CELLULAR RESPIRATION. ADAPTIVE GENE PRODUCTS ARE ONLY REQUIRED UNDER CERTAIN CONDITIONS. GENES FOR PROTEIN DIGESTION ARE TURNED OFF WHEN THERE IS NO PROTEIN AVAILABLE. . Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. RNA can destabilize mRNA. Single-stranded RNA can affect gene expression. Double-stranded RNA can affect gene expression. All of above. . Before their study, it is known that. Single stranded RNA can cause RNA interference. transcriptomic. ) . data analysis. Ståle. . Nygård. , Bioinformatics core facility, OUS/UiO. staaln@ifi.uio.no. Gene expression. Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.. We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g Resources and Gene Set Enrichment Analysis. Genomics Lesson . 8_3. Hardison. resources. 3/24/15. 2. Try it yourself. Go to Gene Expression Omnibus at NCBI. http://. www.ncbi.nlm.nih.gov. /geo/. Choose a dataset of interest, e.g.:. Regulation of Gene Transcription at Promoters. Experimental Analysis of Gene Expression. Statistics Primer. Preprocessing of Data. Differential Expression Analysis. Tue, Nov 19, 2019. 8.1. Regulation of Prokaryotic Gene Transcription.