PPT-V4 – differential gene expression

analysis outliers V2 batch effects V3 data imputation What is measured by microarrays Microarray normalization Differential gene expression DE analysis based on microarray data Detection of outliers. L Ledeganckstraat 35 9000 Ghent Belgium Institute for Agricultural and Fisheries Research ILVO Burg Van Gansberghelaan 115 bus 1 9820 Merelbeke Belgium Corresponding author amaroufiuokacir Abstract Fructan 1 exohydrolase 1 FEH FEH enzymes are involve 486 Benson K 1 1. DIFFERENTIAL GENE EXPRESSION IN CELLS SLOUGHED IN THE URINE IN INTERSTITIAL CYSTITIS SUBJECTS Hypothesis / aims of study We hypothesize that subjects affected with Interstitial Cys limma. package. 20 March 2012. Functional Genomics. Linear regression. Fit a straight line through a set of points such that the distance from the points to the line is minimized. The slope of the line is adjusted to minimize the . Ju. . Han Kim . Division of Biomedical Informatics, Seoul National University College of Medicine, Seoul, Korea, . Presenter: Zhen Gao . 2. Outline. Review of major computational . approaches to facilitate biological interpretation of . What does the operon model attempt to explain?. the coordinated control of gene expression . in bacteria. bacterial resistance to antibiotics. how genes move between homologous regions of DNA. the mechanism of viral attachment to a host cell. Alisha Holloway, PhD. Gladstone Bioinformatics Core Director. What is RNA-seq?. Use of high-throughput sequencing technologies to assess the RNA content of a sample.. Why do an RNA-seq experiment?. Detect . Developmental Genetics. Gilbert 9e – Chapter 2. This chapter is all about making different cells from equivalent DNA sequence..... 1. Every . somatic cell has the complete . genome. . 2. A . small percentage of the genome is . Prokaryotes and eukaryotes precisely regulate gene expression in response to environmental conditions. In multicellular eukaryotes, gene expression regulates development and is responsible for differences in cell types. Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. Identification of a Differentially-expressed Gene in Fatty Liver Acta Biochim Biophys SinVol. 39, No. 9 and 5 females in each group). All of the geese were hatchedconditions. All of the geese had f We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g What is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. RNAseq. data. DE analysis based on . RNAseq. data. RNA-Seq. G-OnRamp Beta Users Workshop. Wilson Leung. 07/2017. Outline. Design considerations for RNA-Seq experiments. Interpret FastQC results. Optimize alignment parameters for HISAT. Assess alignment statistics with CollectRnaSeqMetrics.