PPT-Impact of Human Cytomegalovirus (HCMV) protein US27 on host & viral gene expression

Pearley Chinta and Juliet V Spencer Abstract Methods Results Conclusions HCMV is a widespread pathogen in the general population and can cause severe disease in immunecompromised hosts HCMV manipulates immune responses in several ways one of which includes encoding genes with homology to host chemokine receptors HCMV US27 encodes a chemokinelike receptor that stimulates host gene expression While no chemokine ligand has been identified for US27 it is constitutively active US27 stimulates the gene expression of antioxidant response element ARE regulated genes by activation of the transcription factor nuclear respiratory factor 1 NRF1 The goal of this project is to identify specific host and viral genes that are regulated by US27 Increased expression of antioxidant genes is likely to benefit virus infection and enable more progeny virus to be produced Thus a better understanding of the US27 function has the potential to lead to the development of novel antiviral therapies necessary to treat HCMV infection . Khansa. . Bukhari. Maryum. . Zeb. . History and Definition. Frederick . Twort. (1915) and Felix . d'Herelle. (1917) were the first to recognize bacteriophage.. In the 1930s and after words virologists utilized these viruses as model systems to investigate many aspects of virology. nk. cell evasion functions target mica for . lysosomal. degradation. Audrey . Lullo, Brett . Seaholm. , . Socheata. . Vong. , Jamie . islas. , Cody . Carini. , . Immune Evasion. “Methods used by pathogenic organisms to evade a host’s immune system.”. Adam Stevens, Daniel Hanson, Andrew . Whatmore. , Benoit . Destenaves. , Pierre . Chatelain. , Peter . Clayton. Supplementary Information: . Introduction. In this Supplementary Information we make available additional data that were discussed in the main manuscript. . . Gene therapy: . to correct a . genetic defect . by transferring of a . functional normal copy. of the gene into cells. Examples of diseases caused by genetic defect. Ornithine . transcarbamylase. to correct a . genetic defect . by transferring of a . functional normal copy. of the gene into cells. Examples of diseases caused by genetic defect. Ornithine . transcarbamylase. (OTC deficiency). Many of biopharmaceuticals, especially proteins : produced by recombinant DNA technology using various expression systems. Expression systems : . E. coli. , . Bacillus. , Yeast(. Saccharomyces. . cerevisiae. Anablep. . anablep. . with its “4 eyes”. Upper half of lids look aerially while the lower half looks into the water.. Cells of the two parts of the eye exhibit differential gene expression. Individual bacteria respond to environmental change by regulating their gene expression. Dynamic modeling. Stefan Legewie & Sofya Lipnitskaya. Institute of Molecular Biology, Mainz. What is dynamic model of a biological system?. (g) Comparison/fitting to data. . Iterative cycle of model and experiment . TUMOR SUPRESSOR PROTEINS. ATP. ENZYMES THAT CONTROL CELLULAR RESPIRATION. ADAPTIVE GENE PRODUCTS ARE ONLY REQUIRED UNDER CERTAIN CONDITIONS. GENES FOR PROTEIN DIGESTION ARE TURNED OFF WHEN THERE IS NO PROTEIN AVAILABLE. . SPRING 2019. Biomolecular. Principles: Nucleic Acids and Proteins- part 2 . Lecturer:. Jasmin Sutkovic . 18. .3.2019. Lecture. 2b. Content . DNA . System biology. Water: The medium of Life. Biochemical Energetics . detected by by 46]. The classification of herpes viruses according to their morphology shows a structural similarity for all herpes viruses, which are found in more than 50 different animal species. T CMV pp65 (1-L-11): sc-52401 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe + 00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND Cytomegalo Table 1: Breast Cancer Cell Lines. . Changes in the total amount of hormone receptors will be determined by Western blotting. We expect that HCMV infection will reduce the expression of hormone receptors and decrease the cells’ responsiveness to extracellular stimuli..  which is designed to allow . expression.  (. transcription.  and . translation. ) of the inserted section of . DNA. . The . vector.  carries a . promoter.  (normally inducible) on one side of the .