PPT-Microarray Gene Expression Analysis

Differential expression clustering networks and functional enrichment STEMREM 201 Fall 2012 Aaron Newman PhD 101712 A genomics approach to biology involves A plethora of . dickkopf. 1 . in the bovine . morula.  . Anna C. . Denicol. , Kyle B. Dobbs and Peter J. Hansen. Department of Animal Sciences,. . DH Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, . Mark . Bergland. and Karen . Klyczek. University of Wisconsin-River Falls. . ACUBE Annual Meeting, Lakeland College, . 2012. Case It! Project. URL for . Case It! Home Page:. http. ://caseit.uwrf.edu. Bing Zhang. Department of Biomedical Informatics. Vanderbilt University. bing.zhang@vanderbilt.edu. Overall workflow of gene expression studies. Microarray. Biological question. Experimental . design. . Ciprian . Gheorghe MD PhD.  . . Tyrosine Kinase R. PI3K. PDK. PAK. Src. GRB2. SHC. SOS. Ras. MAPKKK(Raf). MAPKK. -P. MAPKK . (MEK). SR. IP. 3. R. G. PLC. b. a. 1-AR. PKC. DAG. PKA. BMI/IBGP 730 . . Kun Huang. Department of Biomedical Informatics. The Ohio State University. Autumn 2010. Introduction to . gene expression . microarray. A . middle-man’s . approach. Applications of microarray. Alan Ma. O&G Meeting. 12. th. November 2014. Aims. Role of microarray in prenatal testing. How we decipher the microarray results. Common scenarios. Unrelated/incidental findings of significance. Draw 8 boxes on your paper. Gene regulation accounts for some of the phenotypic differences between organisms with similar genes.. 2005-2006. Gene regulation in bacteria. Control of gene expression enables individual bacteria to adjust their metabolism to environmental change. Amy Caudy. Lewis-Sigler Fellow. Outline . Microarray platforms. Uses of microarrays . Labeling approaches . Experimental considerations . Emerging technologies . Hybridization – the fundamental principle of array analysis. - Overview -. Why gene expression analysis?. Quantification of mRNA transcript abundance. High specificity, +/- high through-put. Requires sequence knowledge. Considerations. Experimental question. Species limitations . and . Data Analysis . Roy Williams . PhD . Sanford | Burnham Medical Research Institute . Microarray Revolution. Idea. : measure the amount of. . mRNA. . to see which. . genes. . are being. . expressed. Corresponding authorHaben Fesseha, MVSc, DVM Assistant Professor, Department of Veterinary Surgery and Diagnostic Imaging, School of Veterinary Medicine, Wolaita Sodo University, P. O. Box 138, Wolait 20 18 , Vol. 9 http://www. jcancer .org 22 49 J J o o u u r r n n a a l l o o f f C C a a n n c c e e r r 201 8 ; 9 ( 1 3 ) : 2249 - 2 265 . doi: 10.7150/ jca . 24744 Review Gene Expression Detecti Shilpa. . Kaistha. Department of Microbiology. Institute of Biosciences & Biotechnology. CSJM University, Kanpur. Microarrays can be used in many types of experiments including . Genotyping. epigenetics. MBI401-High throughput Data analysis. Mamta Sagar. Department of Bioinformatics. UIET-IBSBT, CSJM University, Kanpur. 1. INTRODUCTION. Functional genomics involves the analysis of large datasets of information derived from various biological experiments. One such type of large-scale experiment involves monitoring the expression levels of thousands of genes simultaneously under a particular condition,.