PPT-Microarray Data Analysis

Shilpa Kaistha Department of Microbiology Institute of Biosciences amp Biotechnology CSJM University Kanpur Microarrays can be used in many types of experiments including Genotyping epigenetics. Transcriptomics – towards RNASeq Federico M. Giorgi – federico.giorgi@gmail.com Analisi del Genoma e Bioinformatica Corso di Laurea Specialistica in Biotecnologie delle piante Class name – VIH. Course name - ZOO-Biotech. (Rohit). DNA MICROARRAY. Introduction. As we know that for molecular characterisation of any gene/genome, nucleotide sequences are required.. Understanding the genome function by analysing the genes and the ways the genes expressed by the genome have a key importance.. Microarray . Analysis and Omics Technology. Introduction. Approximately humans have 25000 genes. Only . a fraction of these are actively expressed as mRNAs at any one time. . Hybridization between the cDNA reverse transcribed from a biological sample to a pre-designed complementary DNA probe arranged on a slide, or array, is the basis of DNA microarrays.. Amy Caudy. Lewis-Sigler Fellow. Outline . Microarray platforms. Uses of microarrays . Labeling approaches . Experimental considerations . Emerging technologies . Hybridization – the fundamental principle of array analysis. - Overview -. Why gene expression analysis?. Quantification of mRNA transcript abundance. High specificity, +/- high through-put. Requires sequence knowledge. Considerations. Experimental question. Species limitations . Peptide intensity vs m/z. Previous Lecture: . Proteomics Informatics. Gene Expression Analysis (I). This Lecture. Learning Objectives. Microarray experimental details. Microarray data formats. QC analysis and data exploration. and . Data Analysis . Roy Williams . PhD . Sanford | Burnham Medical Research Institute . Microarray Revolution. Idea. : measure the amount of. . mRNA. . to see which. . genes. . are being. . expressed. Outline. Introduction. Two review papers. Quality control (. MetaQC. ). Meta-analysis for detecting differentially expressed genes (. MetaDE. ). Meta-analysis for detecting pathways (. MetaPath. ). 1. Introduction. Corresponding authorHaben Fesseha, MVSc, DVM Assistant Professor, Department of Veterinary Surgery and Diagnostic Imaging, School of Veterinary Medicine, Wolaita Sodo University, P. O. Box 138, Wolait AMB Review 11/2010. Consensus Clustering . (. Monti. et al. 2002). Internal validation method for clustering algorithms.. Stability based technique.. Can be used to compare algorithms or for estimating the number of clusters in the data.. What is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. RNAseq. data. DE analysis based on . RNAseq. data. Cummings CA, Relman DA. Using DNA Microarrays to Study Host-Microbe Interactions. Emerg Infect Dis. 2000;6(5):513-525. https://doi.org/10.3201/eid0605.000511. Presenter: . R2. . 張家甄. Supervisor: . Fellow. . 楊思婷. 醫師. Outline. PART ONE: BASIC . CONCEPTS. 2. . Chromosome . Analysis. INTRODUCTION. CLASSICAL CYTOGENETIC . ANALYSIS. MICROARRAY . MBI401-High throughput Data analysis. Mamta Sagar. Department of Bioinformatics. UIET-IBSBT, CSJM University, Kanpur. 1. INTRODUCTION. Functional genomics involves the analysis of large datasets of information derived from various biological experiments. One such type of large-scale experiment involves monitoring the expression levels of thousands of genes simultaneously under a particular condition,.