PPT-Example data – MALDI-TOF

Peptide intensity vs mz Previous Lecture Proteomics Informatics Gene Expression Analysis I This Lecture Learning Objectives Microarray experimental details Microarray data formats QC analysis and data exploration. Barrett-wilt. , . phd. UW Mass spectrometry/proteomics facility. MASS ANALYZERS AND IONIZATION METHODS. Central components of a mass spectrometer. Vacuum chamber. Ionization source. Mass analyzer/filter. Yi Guo, PharmD. Clinical Pharmacy Manager of Infectious Diseases. Montefiore Medical Center. Albert Einstein College of Medicine. Bronx, NY. September 2015. Disclosures. I . have no relevant financial or nonfinancial relationship(s) with the manufacturers of the antimicrobial agents described or reviewed in this presentation. Presented by:. . Muhammadi. . Ph.D. Scholar. Department of Botany, PMAS-UAAR, RWP.. 2. Introduction. Metabolomics. has largely addressed through the development of sophisticated separation techniques, mass spectrometry approaches, and computational tools . Instrumentation:. Ionization methods and analyzers. Parts of an MS. Ionization Methods. EI. CI. FD. FAB. MALDI. ES. EI (and CI). EI vs CI. FD. http://en.wikipedia.org/wiki/Field_desorption. High voltage applied to metal emitter with carbon . S. pectrometer(MS). 분자가 . MS . 내로 들어가면 분자는 이온화됨과 동시에 더 작은 이온들. (fragments). 로 쪼개진다. . . 쪼개진 이온들은 그들의 질량. /. 전. 하. H. . Kabarowski. , PhD. UAB Metabolomics Workshop. December 2, 2015. O. O. O. OH. O. O. P. OH. H. CH. 3. N. CH. 3. CH. 3. O. HO. HO. O. HO. HO. O. HO. HO. CH. 3. CH. 3. C. H. 3. C. CH. 3. CH. 3. OH. Dilution. Washing. Drop Dialysis. Cation. Exchange. Zip Tips. Typical contaminants in protein/peptide samples. No interference: TFA, formic acid, acetic acid, . . -mercaptoethanol, DTT, HCl, NH. 4. Michael Costello, Ph.D Technical Director – Microbiology ACL Laboratories Mike.costello@advocatehealth.com WSLH Teleconference November 13, 2013 Implementation of MALDI-TOF in Clinical Microbiology ů hmotnostní spektrometrií. C7250. Část . V. Zbyněk Zdráhal. Výzkumná. . skupina. . Proteomika. , CEITEC-MU. Centrální. . laboratoř-Proteomika. , CEITEC-MU. Funkční. . genomika. a . MALDI-TOF. Oft notað fyrir risa-sameindir sem erfitt er að koma í gasfasa.. Fræðilegur Grunnur MALDI-TOF. Sterkur LASER geisli sendur á matrixu, þ.s. Bylgjulengd er háð efni í matrixu.. Eindabrot ráfa inn í rafsvið þar sem þeim er hraðað þannig að. VASwitzerlandCovalX AG-Ausstellungsstrasse 36-CH-8005 Zrich 41/044 585 3961USACovalX Instruments Inc-999 Broadway Suite 305-SaugusBoston MA 01906 1617297-2263France CovalX -146 rue Lo Saignat BP078-33 SEQUENOM MALDI-TOF-MS. Samples were genotyped at INRA on their . Seqeunom. MALDI-TOF mass spectrometer . MALDI-TOF-MS. Matrix assisted laser . desorbption. . ionisation. – time of flight – mass spectrometry. Garrett L. Schey. 1*. , Emily R. Hildebrandt. 2. , Sadie X. H. Novak. 3. , James L. Hougland. 3. , Walter K. Schmidt. 2. , Mark D. Distefano. 1. . 1. . Department of Medicinal Chemistry, University of Minnesota, Minneapolis, Minnesota USA. Buddy Newton, MD FACP. Director of Antimicrobial Stewardship. Washington Regional Medical Center. Fayetteville, AR . jnewton@wregional.com. Disclosures. Speaker’s Bureau for Roche Diagnostics. I have prescribed and occasionally taken antibiotics.