PPT-Introduction to Microarray Data Analysis

BMIIBGP 730 Kun Huang Department of Biomedical Informatics The Ohio State University Autumn 2010 Introduction to gene expression microarray A middlemans approach Applications of microarray. Transcriptomics – towards RNASeq Federico M. Giorgi – federico.giorgi@gmail.com Analisi del Genoma e Bioinformatica Corso di Laurea Specialistica in Biotecnologie delle piante KEMRI Wellcome Largest malaria study of its kind made possible by Arrayjet technology www.Arrayjet.com mail@Arrayjet.com Nearly half the world’s population is at risk of Malaria, and the Plasmodiu Class name – VIH. Course name - ZOO-Biotech. (Rohit). DNA MICROARRAY. Introduction. As we know that for molecular characterisation of any gene/genome, nucleotide sequences are required.. Understanding the genome function by analysing the genes and the ways the genes expressed by the genome have a key importance.. Microarrays. Established. in 2006. as a center . supporting. . functional. . genomics. . research. in . nation and . internationwide. Comprehensive and . state. . of. . the . art. . functional. Microarray . Analysis and Omics Technology. Introduction. Approximately humans have 25000 genes. Only . a fraction of these are actively expressed as mRNAs at any one time. . Hybridization between the cDNA reverse transcribed from a biological sample to a pre-designed complementary DNA probe arranged on a slide, or array, is the basis of DNA microarrays.. Amy Caudy. Lewis-Sigler Fellow. Outline . Microarray platforms. Uses of microarrays . Labeling approaches . Experimental considerations . Emerging technologies . Hybridization – the fundamental principle of array analysis. transcriptomic. ) . data analysis. Ståle. . Nygård. , Bioinformatics core facility, OUS/UiO. staaln@ifi.uio.no. Gene expression. Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.. Peptide intensity vs m/z. Previous Lecture: . Proteomics Informatics. Gene Expression Analysis (I). This Lecture. Learning Objectives. Microarray experimental details. Microarray data formats. QC analysis and data exploration. Outline. Introduction. Two review papers. Quality control (. MetaQC. ). Meta-analysis for detecting differentially expressed genes (. MetaDE. ). Meta-analysis for detecting pathways (. MetaPath. ). 1. Introduction. Corresponding authorHaben Fesseha, MVSc, DVM Assistant Professor, Department of Veterinary Surgery and Diagnostic Imaging, School of Veterinary Medicine, Wolaita Sodo University, P. O. Box 138, Wolait The Benefits of Reading Books,Most people read to read and the benefits of reading are surplus. But what are the benefits of reading. Keep reading to find out how reading will help you and may even add years to your life!.The Benefits of Reading Books,What are the benefits of reading you ask? Down below we have listed some of the most common benefits and ones that you will definitely enjoy along with the new adventures provided by the novel you choose to read.,Exercise the Brain by Reading .When you read, your brain gets a workout. You have to remember the various characters, settings, plots and retain that information throughout the book. Your brain is doing a lot of work and you don’t even realize it. Which makes it the perfect exercise! Cummings CA, Relman DA. Using DNA Microarrays to Study Host-Microbe Interactions. Emerg Infect Dis. 2000;6(5):513-525. https://doi.org/10.3201/eid0605.000511. Presenter: . R2. . 張家甄. Supervisor: . Fellow. . 楊思婷. 醫師. Outline. PART ONE: BASIC . CONCEPTS. 2. . Chromosome . Analysis. INTRODUCTION. CLASSICAL CYTOGENETIC . ANALYSIS. MICROARRAY . V2: . data imputation . V3: batch effects. What is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. RNAseq.