versus virus evolution VMioulet AMorris BWood BThapa AGray LHenry NJKnowles JWadsworth DPKing WHY Serotyping done using pAg ELISA In the last few years this test failed to serotype a number ID: 1046934
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1. Antigen-detection ELISA performance versus virus evolutionV.Mioulet, A.Morris, B.Wood, B.Thapa, A.Gray, L.Henry, N.J.Knowles, J.Wadsworth, D.P.King
2. WHY?Serotyping done using pAg-ELISAIn the last few years this test failed to serotype a number of samplesIs there a pattern?Samples logged and sets identifiedOACSAT 1SAT 2SAT 3ASIA 1SVDVCtrls
3. EXAMPLE: O/CATHAY%No issues in serotyping using polyclonal Ag-ELISALow to borderline OD values on standard Ag-ELISA / Confirmation may have been required using the integrin-mAb ELISAAdditional ELISA required for determination of serotype
4. Best practice to not rely on a single test for serotypingTests in use should be subjected to ongoing validation against circulating field strainsHighlights the importance of ongoing work to develop and validate new assays and reagents to detect current circulating strains of FMDVCONCLUSIONS