Biow are Microorgan ism Ha emophilus inf uenzae Xen In vitro Characteristics Genetic Ch ara teri stics Haemop hilus i fluenzae X n wa s deriv d fro th parental strain H
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Biow are Microorgan ism Ha emophilus inf uenzae Xen In vitro Characteristics Genetic Ch ara teri stics Haemop hilus i fluenzae X n wa s deriv d fro th parental strain H

influen zae Rd TCC 5 1907 H influenzae Xe n21 posse sse a st able copy of Photorhabd us l mine scen s lux DABE operon at a single integration site on the b acterial chro so 21 shoul be store at 80 C Gro th Cha ac teristi s H influenzae Xen21 nor l

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Biow are Microorgan ism Ha emophilus inf uenzae Xen In vitro Characteristics Genetic Ch ara teri stics Haemop hilus i fluenzae X n wa s deriv d fro th parental strain H




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Presentation on theme: "Biow are Microorgan ism Ha emophilus inf uenzae Xen In vitro Characteristics Genetic Ch ara teri stics Haemop hilus i fluenzae X n wa s deriv d fro th parental strain H"— Presentation transcript:


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Biow are Microorgan ism Ha emophilus inf uenzae Xen21 In vitro Characteristics Genetic Ch ara teri stics Haemop hilus i fluenzae X n21 wa s deriv d fro th parental strain H. influen zae Rd ( TCC 5 1907). H. influenzae Xe n21 posse sse a st able copy of Photorhabd us l mine scen s lux DABE operon at a single integration site on the b acterial chro so . 21 shoul be store at -80 C Gro th Cha ac teristi s H. influenzae Xen21 nor ll grows well in rich dia ch as Brain-Heart Infus on (BHI), s itably supple ented with required cofactors, ni cotina adenine din cle tide (NAD, 2-10 g/

l) and he n (10 g/ l), and inc ubated at 37 C, with 5% CO . Optional Antibi tic Selection H. influenzae Xen21 can b grown on chocolate agar plates supple ted with 50 g/ l kan cin. Colonial Morp hology On chocol ate a ar plates, H. influenzae Xen2 1 colonie are gray ish p ff and slightly transpare t, with little od or. Appear s as small (ap rox 0.5 mm), circular, gray colonie . Gro th Cu rve Log-phase growth can be a eved after 3 t 4 hours of subcult re in rich media suc as Brain-He art Infusio (BHI), suitably supple ented with he n and NA D, incubated at 37 with aeratio n in culture tub s.

For th e above broth culture co nditions, an ab sorbanc asure ent a 600n (again st a rich dia blank) of 0.6 is roughl equivalent t 1.5x10 cfu of H. influenzae Xen 21 and the rel tive light inte nsity is 1.8 photons/ /cell 0. 0. 10 10 10 10 10 246 H. i X 1 60 0 Xe 1O 00 s/ se OD s/ se Ti ( Bio hemica l Pro ile Carbohy te Fermentation Tests Test Xe 2 Gl uc Suc nn Xy Biotype Tests Test Xe 2 In Pr duct on - Ornithine Deca rboxylase - Hemin (X fact or) and NA D (V fact or) De pe ndency H. in flu en za e Xen 21 di d depe enc for b hem n and c ng s eci at Antib io tic Suscep tib ility Disk Dif usi ta

Di sk di si t st s were pe rf orm d a ccor g t thods outlined in the NC CLS Approve Standard M2 -A 7. Kirby Bauer Disk Diff usio Test Sensitive Interme diate Resistan t cil 100 cin 5 ram phe ni col 1 Ery hr om 50 Tetracycline 5 Contact Information: If y ou ve an questions reg rd ing th es e cell lin s, please con act Caliper at 508- 497-6592 or e-ma il: reagen ts@c aliperls .com