PPT-RNA-Seq Primer

Understanding the RNASeq evidence tracks on the GEP UCSC Genome Browser Wilson Leung 082016 Introduction to RNASeq RNASeq Massively parallel RNA Seq uencing using second or third generation sequencing technologies. . Analysis. Team . McGill . University. and . Genome. . Quebec. Innovation Center. bioinformatics.service@mail.mcgill.ca. RNAseq. . analysis. 2. Module #: Title of Module. Why sequence RNA?. Functional studies. John M. Rosenfeld, Ph.D.. External Innovation Manager. EMD Millipore. Temecula, CA . Genomics & Pharmacogenomics 2015. Confidential and Property of EMD Millipore Corporation. Chromatin Biology has come a long way…. Outline. RNA Immuno-Precipitation (RIP). NGS on RIP & its alternatives. Alternate splicing. Transcription as a graph. Distribution of tags in exons. Pipeline on RIP-seq dataset. RNA Immuno-Precipitation (RIP). July 13, 2015. Goal of the . course. To be able to effectively design, and interpret genomic studies of gene expression.. We will focus on RNA-seq, but the class will provide a foothold into other functional genomics assays.. Bioinformatics . Workshop:. RNA . Seq. using Galaxy. Typical . RNA_Seq. Project Work Flow. Sequencing. Tissue Sample. . Cufflinks. . TopHat. FASTQ file. . QC. . Gene/Transcript/. Exon. Expression. John M. Rosenfeld, Ph.D.. External Innovation Manager. EMD Millipore. Temecula, CA . Genomics & Pharmacogenomics 2015. Confidential and Property of EMD Millipore Corporation. Chromatin Biology has come a long way…. Jenny . Wu. Outline. Goals : Practical guide to NGS data processing. Bioinformatics in NGS data analysis. Basics: terminology, data formats, general workflow etc.. Data Analysis Pipeline. Sequence QC and preprocessing. Alisha Holloway, PhD. Gladstone Bioinformatics Core Director. What is RNA-seq?. Use of high-throughput sequencing technologies to assess the RNA content of a sample.. Why do an RNA-seq experiment?. Detect . UNIT . 5. Gene expression – A misnomer ?. In reality, gene expression can only be quantified by looking at protein products in the cell (. via. proteomic approaches).. T. he . term has been co-opted to describe differences in transcript (mRNA) levels.. Analysis 2015. - RNA-sequencing . Jonas Andreas . Sibbesen. & Lasse Maretty. Bioinformatics Centre, University of Copenhagen . Bioinformatics Centre. Next Generation Sequencing Analysis 2015 - RNA-seq. Barbara Radovani. Genomics. . Master . of. Advanced . genetics. , UAB. Transcriptome. . Source. : https://www.genome.gov/13014330/transcriptome-fact-sheet/. The transcriptome, the entire repertoire of transcripts in. Jeremy Buhler. for GEP Alumni Workshop. RNA-Seq Pipeline for Expression Analysis. RNA Source. 37251. 20653. 9827. 5121. RNA-Seq Read Count . per Transcript. Map reads to transcripts. RNA Abundance. Jessica . Podnar. Outline. Overview of RNA-. Seq. methods. Tag-. Seq. SOP. Cost Breakdown. Submitting Samples. RNA-. Seq. Methods. RNA-. Seq. Methods. GSAF has primarily used . two methods for . RNA-. John Kenny. Centre for Genomic Research, University of Liverpool.. CGR. CGR. Experimental Design, Bioinformatics.. Library production/sequence generation:. RNA-Seq, . SAGE, . Fragment, . Mate-pair, .