PPT-RNA-seq: Quantifying the Transcriptome

Author : karlyn-bohler | Published Date : 2017-06-04

Alisha Holloway PhD Gladstone Bioinformatics Core Director What is RNAseq Use of highthroughput sequencing technologies to assess the RNA content of a sample Why

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RNA-seq: Quantifying the Transcriptome: Transcript

Alisha Holloway PhD Gladstone Bioinformatics Core Director What is RNAseq Use of highthroughput sequencing technologies to assess the RNA content of a sample Why do an RNAseq experiment Detect . Whole . brain . RNA-. Seq. Data . from . Sanger Institute Mouse Genomes . Project [Keane et al. 2011]. Synthetic hybrids with different levels of . heterozygosity. generated by pooling reads from C57/BL6 and four other strains. John M. Rosenfeld, Ph.D.. External Innovation Manager. EMD Millipore. Temecula, CA . Genomics & Pharmacogenomics 2015. Confidential and Property of EMD Millipore Corporation. Chromatin Biology has come a long way…. A (soon to be outdated) Tutorial. A Brief History of Sequencing and Gene Expression. Limitations of Sanger Sequencing. Low throughput. Inconsistent base quality. Expensive. Not quantitative. Frederick “. Sequence Differences in the. Human . Transcriptome. Mingyao. Li. , Isabel . X. Wang. , . Yun. Li, . Alan . Bruzel. , . Allison L. Richards. ,. Jonathan M. . Toung. , . Vivian G. . Cheung. Mahnaz. . Seq. . and Transcriptome Analysis. Hands – on activities (Fun with UNIX!). PowerPoint: Jessica . Kirkpatrick and Casey Hanson. RNA-. Seq. Lab | Jessica Kirkpatrick | 2015. 1. Exercise. Use the . UNIT . 5. Gene expression – A misnomer ?. In reality, gene expression can only be quantified by looking at protein products in the cell (. via. proteomic approaches).. T. he . term has been co-opted to describe differences in transcript (mRNA) levels.. Joel Parker, Ph.D.. LCCC Biomedical Informatics. UNCseq. : . Cancer genome analysis of 1000 UNC Hospital patients. TCGA: Processed and distributed 8K cancer . transcriptomes. (>1PB). Cancer Survivorship . BMI/CS 776 . www.biostat.wisc.edu/bmi776/. Spring 2022. Daifeng. Wang. daifeng.wang@wisc.edu. These slides, excluding third-party material, are licensed under . CC BY-NC 4.0. by Mark Craven, Colin Dewey, Anthony . Barbara Radovani. Genomics. . Master . of. Advanced . genetics. , UAB. Transcriptome. . Source. : https://www.genome.gov/13014330/transcriptome-fact-sheet/. The transcriptome, the entire repertoire of transcripts in. Jeremy Buhler. for GEP Alumni Workshop. RNA-Seq Pipeline for Expression Analysis. RNA Source. 37251. 20653. 9827. 5121. RNA-Seq Read Count . per Transcript. Map reads to transcripts. RNA Abundance. Genomics Lesson . 8_1. Hardison. 3/16/15. 1. transcriptomes. 3/16/15. 2. Transcriptome. . All the DNA that is transcribed in all the cells in an organism. Protein-coding genes. Both primary transcript and mature mRNA. Analysis. Jessica Holmes. High Performance Biological Computing (HPCBio). Roy J. Carver Biotechnology Center. General Outline. Getting the RNA-Seq data: from RNA -> Sequence data. Experimental and practical considerations. BIOINFORMATICA. per il CLM in BIOLOGIA EVOLUZIONISTICA. Scuola di Scienze, Università di Padova. Prof. STEFANIA BORTOLUZZI. Outline. Transcriptomics. today. RNA-. seq. features and advantages. Transcriptome. BIOINFORMATICA. per il CLM in BIOLOGIA EVOLUZIONISTICA. Scuola di Scienze, Università di Padova. Prof. STEFANIA BORTOLUZZI. Outline. Transcriptomics. today. RNA-. seq. features and advantages. Transcriptome.

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