PPT-Introduction to RNA- seq

Author : trish-goza | Published Date : 2018-10-09

Joel Parker PhD LCCC Biomedical Informatics UNCseq Cancer genome analysis of 1000 UNC Hospital patients TCGA Processed and distributed 8K cancer transcriptomes

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Introduction to RNA- seq: Transcript


Joel Parker PhD LCCC Biomedical Informatics UNCseq Cancer genome analysis of 1000 UNC Hospital patients TCGA Processed and distributed 8K cancer transcriptomes gt1PB Cancer Survivorship . July 13, 2015. Goal of the . course. To be able to effectively design, and interpret genomic studies of gene expression.. We will focus on RNA-seq, but the class will provide a foothold into other functional genomics assays.. Sequence Differences in the. Human . Transcriptome. Mingyao. Li. , Isabel . X. Wang. , . Yun. Li, . Alan . Bruzel. , . Allison L. Richards. ,. Jonathan M. . Toung. , . Vivian G. . Cheung. Mahnaz. . Understanding the RNA-Seq evidence tracks on . the GEP UCSC Genome Browser. Wilson Leung. . 08/2016. Introduction to RNA-Seq. RNA-Seq: Massively parallel . RNA. . Seq. uencing using second or third generation sequencing technologies. Alisha Holloway, PhD. Gladstone Bioinformatics Core Director. What is RNA-seq?. Use of high-throughput sequencing technologies to assess the RNA content of a sample.. Why do an RNA-seq experiment?. Detect . RNA-. seq. BMI 877. Spring . 2017. Colin Dewey. colin.dewey@wisc.edu. Overview. Some motivation: axolotl. RNA-. seq. . technology. The . RNA-. seq. . quantification problem. Generative probabilistic models and Expectation-Maximization for the quantification task. data for Peptide and Protein Identification. ABRF 2013, Palm Springs, CA. 3/02-05/2013. iPRG2013 Study:. DESIGN. Study Goals. Primary. : Evaluate how many extra peptide sequence identifications can be determined using databases derived from RNA-. Hailun Wang. , Pak Sham, Tiejun Tong and . Herbert Pang. Rocky 2019. December 7. th. (Saturday). 2. W. hy . scRNA. -Seq data. Propose a pathway-based analytic framework using Random Forests. Identify discriminative functional pathways related to cellular heterogeneity . Analysis 2015. - RNA-sequencing . Jonas Andreas . Sibbesen. & Lasse Maretty. Bioinformatics Centre, University of Copenhagen . Bioinformatics Centre. Next Generation Sequencing Analysis 2015 - RNA-seq. mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. High Throughput Sequencing. TexPoint fonts used in EMF: . A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. David Tse. U.C. Berkeley. ITA . Feb , 2013. Research supported by NSF Center for Science of Information.. Jeremy Buhler. for GEP Alumni Workshop. RNA-Seq Pipeline for Expression Analysis. RNA Source. 37251. 20653. 9827. 5121. RNA-Seq Read Count . per Transcript. Map reads to transcripts. RNA Abundance. Jessica . Podnar. Outline. Overview of RNA-. Seq. methods. Tag-. Seq. SOP. Cost Breakdown. Submitting Samples. RNA-. Seq. Methods. RNA-. Seq. Methods. GSAF has primarily used . two methods for . RNA-. Jessica . Podnar. Outline. RNA. Quality check of the RNA. Types of RNA used for library prep. Library Prep Methods used in the GSAF. Ligation Based. dUTP. method. Tag-. Seq. RNA, the Starting Material . John Kenny. Centre for Genomic Research, University of Liverpool.. CGR. CGR. Experimental Design, Bioinformatics.. Library production/sequence generation:. RNA-Seq, . SAGE, . Fragment, . Mate-pair, .

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