PPT-Next Generation Sequencing

Analysis 2015 RNAsequencing Jonas Andreas Sibbesen amp Lasse Maretty Bioinformatics Centre University of Copenhagen Bioinformatics Centre Next Generation Sequencing Analysis 2015 RNAseq. Next-generation shotgun sequencing approaches require sequencing every base in a sample several times for two reasons: DNA Sequencing. Platforms:. Evolving Tools for . Cancer Research. Norma Neff. Bioengineering / Quake Lab. Sequencing Core Director. Stem Cell Institute. SIM1 G1115 / G0821. nfneff@stanford.edu. Emulsion PCR-based Sequencing Technologies. Dan . Russell. The past, present, and future of DNA . sequencing*. Dan . Russell. *DNA sequencing:. D. etermining the number and order of nucleotides that make up a given molecule of DNA.. (Relevant) Trivia. Assembly, and Alignment Methods . Andy Nagar. Agenda. Background. Next Generation Sequencing. Sequence Assembly. Sequence Alignment. Traditional Alignment Algorithms. Next Generation Alignment Algorithms. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . :. informatics & software aspects. Gabor T. Marth. Boston College Biology . Department. BI543 Fall 2013. January 29, 2013. Traditional DNA sequencing. Genetics of living organisms. DNA. Chromosomes. Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Ion . Mandoiu. Computer Science and Engineering Department. University of Connecticut. Outline. Background on high-throughput sequencing. Identification of tumor-specific . epitopes. Estimation of gene and . Next-generation sequencing technology. Outline. First generation sequencing. Next generation sequencing (current). AKA: . Second generation sequencing. Massively parallel sequencing. Ultra high-throughput sequencing. Trajectory Trends Breakfast. July 2016. What future for Generation Z?. Why Generation Z?. 2016. By 2029, Gen Z will be the largest generational cohort. Source: Office of National Statistics, 2014 based National Population Projections. Hardison. Genomics 3_1. 1. 1/20/14. nucleo. s. ides. A nucleo. t. ide has one or more phosphates attached to the 5’ hydroxyl of the (deoxy)ribose. Building blocks of DNA. 1/20/14. 2. Structure of a dinucleotide. Alexandre . Gillet-. Markowska. Alexandre.gillet-markowska@upmc.fr. Gilles Fischer Team – . Biology. of . Genomes. . UMR7238. Laboratory . of Computational and Quantitative Biology. Université Pierre et Marie-Curie, Paris. the DNA sequencing?. DNA sequencing determines the order of the four chemical building blocks - called "bases" - that make up the DNA molecule. . The . sequence tells scientists the kind of genetic information that is carried in a particular DNA segment.