PPT-Introduction To Next Generation Sequencing (NGS) Data Anal

Author : karlyn-bohler | Published Date : 2017-04-06

Jenny Wu Outline Goals Practical guide to NGS data processing Bioinformatics in NGS data analysis Basics terminology data formats general workflow etc Data Analysis

Presentation Embed Code

Download Presentation

Download Presentation The PPT/PDF document "Introduction To Next Generation Sequenc..." is the property of its rightful owner. Permission is granted to download and print the materials on this website for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.

Introduction To Next Generation Sequencing (NGS) Data Anal: Transcript

Jenny Wu Outline Goals Practical guide to NGS data processing Bioinformatics in NGS data analysis Basics terminology data formats general workflow etc Data Analysis Pipeline Sequence QC and preprocessing. Next-generation shotgun sequencing approaches require sequencing every base in a sample several times for two reasons: Next Generation Sequencer Applications. DeNovo Sequencing. Resequencing, Comparative Genomics. Global SNP Analysis. Gene Expression Analysis. Methylation Studies. ChIP . Sequencing-transcription factors, histones, polymerases. Assembly, and Alignment Methods . Andy Nagar. Agenda. Background. Next Generation Sequencing. Sequence Assembly. Sequence Alignment. Traditional Alignment Algorithms. Next Generation Alignment Algorithms. Elaine R. . Mardis. – 11 . February. . 2008. Washington School of Medicine, Genome Sequencing Center.. Presented by . Jacob . Juhn. “If one accepts that the fundamental pursuit of genetics is to determine the genotypes that explain phenotypes, the meteoric increase of DNA sequence information applied toward that pursuit has nowhere to go but up.”. Jenny Wu. UCI Genomics High Throughput Facility. Outline. Goals : Practical guide to NGS data processing. Bioinformatics in NGS data analysis. Basics: terminology, data file formats, general workflow . Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Hardison. Genomics . 3_2. 1. 1/20/14. Second generation sequencing. Michael . Metzker. review. (2010) Nature Reviews Genetics . 11. : 31-46. 1/20/14. 2. Two generations of sequencing technology. Feature. . Nearly . all modern DNA sequencing procedures require a concentrated amount of . single-stranded . DNA known as a . template. . A template is simply a piece of DNA of . sufficient . length and quality to allow for its sequencing (most sequencing methods . Next-generation sequencing technology. Outline. First generation sequencing. Next generation sequencing (current). AKA: . Second generation sequencing. Massively parallel sequencing. Ultra high-throughput sequencing. Analysis 2015. - RNA-sequencing . Jonas Andreas . Sibbesen. & Lasse Maretty. Bioinformatics Centre, University of Copenhagen . Bioinformatics Centre. Next Generation Sequencing Analysis 2015 - RNA-seq. Hardison. Genomics 3_1. 1. 1/20/14. nucleo. s. ides. A nucleo. t. ide has one or more phosphates attached to the 5’ hydroxyl of the (deoxy)ribose. Building blocks of DNA. 1/20/14. 2. Structure of a dinucleotide. Cynthia N Perry, PhD. Assistant Academic Dean of Admissions. Assistant Professor, Molecular Medicine. Department of Medical Education. Foster School of Medicine. https://. youtu.be. /KiQgrK3tge8. Genomics and Healthcare. Assistant Professor. Department of Medicine. Division of Pulmonary Sciences & Critical Care Medicine. What I do:. Perform high-throughput data analysis for . the scientific . community; microarray and .

Download Document

Here is the link to download the presentation.
"Introduction To Next Generation Sequencing (NGS) Data Anal"The content belongs to its owner. You may download and print it for personal use, without modification, and keep all copyright notices. By downloading, you agree to these terms.