Cynthia N Perry PhD Assistant Academic Dean of Admissions Assistant Professor Molecular Medicine Department of Medical Education Foster School of Medicine https youtube KiQgrK3tge8 Genomics and Healthcare ID: 920849
Download Presentation The PPT/PDF document "Emerging Medical Technologies: Next Gen ..." is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
Emerging Medical Technologies: Next Gen Sequencing
Cynthia N Perry, PhDAssistant Academic Dean of AdmissionsAssistant Professor, Molecular MedicineDepartment of Medical EducationFoster School of Medicine
Slide2https://
youtu.be/KiQgrK3tge8
Genomics and Healthcare
Slide3Human Genome
3 billion bases arrayed in a unique order, with ~20,000 genes that direct the synthesis of all the proteins
~1/1,000,000
th
of the Human Genome
Polymorphisms or SNPs which differ between people
May influence traits
Possible medical importance
Slide4History of DNA Sequencing
1st Generation = Sanger Sequencing2 reads (forward & reverse)
2
nd
Generation = Next Generation Sequencing
Millions of reads
3
rd Generation = Single Molecule Sequencing
Slide5Sanger Sequencing
Slide6Sanger Sequencing
Slide7From Sanger to
Next-Gen Sequencing
Slide8Next Generation Sequencing
Slide9Applications IN MEDICINE
Slide10Applications in Medicine-
Infectious diesease
Clinical Test for TB Patient
Take a sputum sample
Begin culture
ID drug susceptibility
ID by NGS
Slide11Applications in Medicine -
Cancer
Slide12Applications in Medicine -
prenatal care
Slide13Broad Applications
Paternity
testing
Wildlife conservation
Herbal supplement validation
Ancestral migration patterns
Origins of traditional medicine
Slide14Portable Sequencer (
MinION)
P
ocket-sized
, portable device for biological
analysis
Up to 512
nanopore channelsSimple 10-min sample prep availableReal-time analysis for rapid, efficient workflows
Adaptable to direct DNA or RNA
sequencing
Slide15Portable Sequencer (MinION
)
Slide16Next Generation Sequencing
In the palm of your hand
Practical module
Miki Wang, Cynthia Perry,
and Jorge Cervantes