PPT-The impact of next-generation sequencing technology of gene

Elaine R Mardis 11 February 2008 Washington School of Medicine Genome Sequencing Center Presented by Jacob Juhn If one accepts that the fundamental pursuit of genetics is to determine the genotypes that explain phenotypes the meteoric increase of DNA sequence information applied toward that pursuit has nowhere to go but up. Assembly, and Alignment Methods . Andy Nagar. Agenda. Background. Next Generation Sequencing. Sequence Assembly. Sequence Alignment. Traditional Alignment Algorithms. Next Generation Alignment Algorithms. Gene Expression . Next-Generation . DNA Sequencing Technology. Stuart M. Brown, Ph.D. This Lecture. Learning Objectives. Illumina Sequencing Technology: . shotgun, amplicon, selected. Quality Scores. 16S rRNA gene sequencing. Sequencing of material from near the hot spring vent source (core 1) resulted in a metage , both members of the phylum Ignavibacteriae. References: [1] Kozubal, M. A. et al. Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. (very) large datasets. 5/23/17. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. Hardison. Genomics . 3_2. 1. 1/20/14. Second generation sequencing. Michael . Metzker. review. (2010) Nature Reviews Genetics . 11. : 31-46. 1/20/14. 2. Two generations of sequencing technology. Feature. (very) large datasets. 5/24/18. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. Next-generation sequencing technology. Outline. First generation sequencing. Next generation sequencing (current). AKA: . Second generation sequencing. Massively parallel sequencing. Ultra high-throughput sequencing. Analysis 2015. - RNA-sequencing . Jonas Andreas . Sibbesen. & Lasse Maretty. Bioinformatics Centre, University of Copenhagen . Bioinformatics Centre. Next Generation Sequencing Analysis 2015 - RNA-seq. Hierarchical genome shotgun HGS – Human Genome Project. “map first, sequence second. ”. clone-by-clone … cloning is performed twice (BAC, plasmid). Sequencing strategies. Whole genome shotgun WGS – Celera. Cynthia N Perry, PhD. Assistant Academic Dean of Admissions. Assistant Professor, Molecular Medicine. Department of Medical Education. Foster School of Medicine. https://. youtu.be. /KiQgrK3tge8. Genomics and Healthcare. Assistant Professor. Department of Medicine. Division of Pulmonary Sciences & Critical Care Medicine. What I do:. Perform high-throughput data analysis for . the scientific . community; microarray and . Igor Ulitsky. “the . branch of genetics that studies organisms in terms of their genomes (their full DNA sequences. )”. Computational genomics in TAU. Ron Shamir’s lab – focus on gene expression and regulatory networks.