PPT-Next-Generation Sequencing: Challenges and Opportunities

Author : alida-meadow | Published Date : 2018-01-08

Ion Mandoiu Computer Science and Engineering Department University of Connecticut Outline Background on highthroughput sequencing Identification of tumorspecific

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Next-Generation Sequencing: Challenges and Opportunities: Transcript

Ion Mandoiu Computer Science and Engineering Department University of Connecticut Outline Background on highthroughput sequencing Identification of tumorspecific epitopes Estimation of gene and . Opportunities for . Small . Credit . Unions . Office of Small Credit Union Initiatives. Martha Ninichuk, Deputy Director. Presentation Title. 2. Many small credit unions are struggling to survive and thrive. Next-generation shotgun sequencing approaches require sequencing every base in a sample several times for two reasons: Phil Bradley. http://www.philb.com. Personal Experiences of Web 2.0. Who am I?. ‘Freelance librarian’ AKA Internet Consultant. Trainer. Writer. Speaker. ‘One man band’. I need…. To keep up to date with news. Assembly, and Alignment Methods . Andy Nagar. Agenda. Background. Next Generation Sequencing. Sequence Assembly. Sequence Alignment. Traditional Alignment Algorithms. Next Generation Alignment Algorithms. ARE YOU HEARING THE . VOICE OF INDUSTRY?. Etienne SICARD. Professor. etienne.sicard@insa-toulouse.fr. INSA TOULOUSE - FRANCE. . www.etienne-sicard.fr. www.ic-emc.org. Home of Rugby (& INSA engineer). Elaine R. . Mardis. – 11 . February. . 2008. Washington School of Medicine, Genome Sequencing Center.. Presented by . Jacob . Juhn. “If one accepts that the fundamental pursuit of genetics is to determine the genotypes that explain phenotypes, the meteoric increase of DNA sequence information applied toward that pursuit has nowhere to go but up.”. Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Hardison. Genomics . 3_2. 1. 1/20/14. Second generation sequencing. Michael . Metzker. review. (2010) Nature Reviews Genetics . 11. : 31-46. 1/20/14. 2. Two generations of sequencing technology. Feature. . Nearly . all modern DNA sequencing procedures require a concentrated amount of . single-stranded . DNA known as a . template. . A template is simply a piece of DNA of . sufficient . length and quality to allow for its sequencing (most sequencing methods . Next-generation sequencing technology. Outline. First generation sequencing. Next generation sequencing (current). AKA: . Second generation sequencing. Massively parallel sequencing. Ultra high-throughput sequencing. Analysis 2015. - RNA-sequencing . Jonas Andreas . Sibbesen. & Lasse Maretty. Bioinformatics Centre, University of Copenhagen . Bioinformatics Centre. Next Generation Sequencing Analysis 2015 - RNA-seq. Hardison. Genomics 3_1. 1. 1/20/14. nucleo. s. ides. A nucleo. t. ide has one or more phosphates attached to the 5’ hydroxyl of the (deoxy)ribose. Building blocks of DNA. 1/20/14. 2. Structure of a dinucleotide. Cynthia N Perry, PhD. Assistant Academic Dean of Admissions. Assistant Professor, Molecular Medicine. Department of Medical Education. Foster School of Medicine. https://. youtu.be. /KiQgrK3tge8. Genomics and Healthcare.

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