PPT-Next-generation sequencing and PBRC

Next Generation Sequencer Applications DeNovo Sequencing Resequencing Comparative Genomics Global SNP Analysis Gene Expression Analysis Methylation Studies ChIP Sequencingtranscription factors histones polymerases. Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Ion . Mandoiu. Computer Science and Engineering Department. University of Connecticut. Outline. Background on high-throughput sequencing. Identification of tumor-specific . epitopes. Estimation of gene and . Hardison. Genomics . 3_2. 1. 1/20/14. Second generation sequencing. Michael . Metzker. review. (2010) Nature Reviews Genetics . 11. : 31-46. 1/20/14. 2. Two generations of sequencing technology. Feature. Kalicki. Heli. J. Roy, RD, PhD. Pennington Biomedical Research Center. Key thoughts. Several . large . studies show that alcohol consumption results in a U-shaped curve. Both abstainers and heavy . drinkers have . Division . of Education. Pennington Biomedical Research Center. Types of tea. Green tea is one of four types of tea that come from the plant . Camellia . Sinensis. . The types include:. White Tea. Green Tea. Pennington Biomedical Research Center. LSU . AgCenter. The DASH Diet Plan. The DASH diet plan. Hypertension prevalence. What is blood pressure. Why is . high blood pressure important?. How it affects the organ systems.  . Nearly . all modern DNA sequencing procedures require a concentrated amount of . single-stranded . DNA known as a . template. . A template is simply a piece of DNA of . sufficient . length and quality to allow for its sequencing (most sequencing methods . Heli. J. Roy, PhD, RD, MBA. Pennington Biomedical Research Center. 5/20/2010. PBRC. 1. Stress is the body’s response . to a . physical, chemical, emotional, or environmental . stress.. Stress reaction includes physiological changes in the body. . Pennington Biomedical Research Center. LSU . AgCenter. The DASH Diet Plan. The DASH diet plan. Hypertension prevalence. What is blood pressure. Why is . high blood pressure important?. How it affects the organ systems. J. Roy, PhD, MBA, RD. Pennington Biomedical Research Center. Depression. Depression is associated with a lack of motivation. This can lead to reduced physical activity, poor eating habits and weight gain.. :. Challenges and Opportunities in the era of Precision Medicine. Dr. Paul . Keown, . 2016. Statement of Conflict & Collaboration. Therapeutics collaborations. Novartis, Roche, . Astellas. , Shire. Genus . Vaccinium. , section . Cyanoccus. .. Blueberries have many health benefits. . Blueberries have antioxidant compounds that may delay chronic diseases.. Blueberries are eaten fresh and are used in cooking.. Alexandre . Gillet-. Markowska. Alexandre.gillet-markowska@upmc.fr. Gilles Fischer Team – . Biology. of . Genomes. . UMR7238. Laboratory . of Computational and Quantitative Biology. Université Pierre et Marie-Curie, Paris.