PPT-Next Generation Sequencing of HLA

Challenges and Opportunities in the era of Precision Medicine Dr Paul Keown 2016 Statement of Conflict amp Collaboration Therapeutics collaborations Novartis Roche Astellas Shire. Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Eric T. Weimer, PhD, D(ABMLI). CONFLICT OF . INTEREST. I . have financial relationship(s) with: . Advisory Board. , Illumina. NGS Implementation at UNC. Received ASHI accreditation in . August . 2015. Fernández-Viña. Histocompatibility, . Immunogenetics. and Disease Profiling Laboratory. Stanford Blood Center. 2. Applications of NGS in Immunogenetics. HLA Typing. KIR Typing. Blood Group Typing. Hardison. Genomics . 3_2. 1. 1/20/14. Second generation sequencing. Michael . Metzker. review. (2010) Nature Reviews Genetics . 11. : 31-46. 1/20/14. 2. Two generations of sequencing technology. Feature.  . Nearly . all modern DNA sequencing procedures require a concentrated amount of . single-stranded . DNA known as a . template. . A template is simply a piece of DNA of . sufficient . length and quality to allow for its sequencing (most sequencing methods . Next-generation sequencing technology. Outline. First generation sequencing. Next generation sequencing (current). AKA: . Second generation sequencing. Massively parallel sequencing. Ultra high-throughput sequencing. Analysis 2015. - RNA-sequencing . Jonas Andreas . Sibbesen. & Lasse Maretty. Bioinformatics Centre, University of Copenhagen . Bioinformatics Centre. Next Generation Sequencing Analysis 2015 - RNA-seq. Pawan. Sharma. Assistant Professor. Mangalayatan University, India. Japanese encephalitis virus (JEV) is a flavivirus, . family . Flaviviridea. Causes disease to human beings: . Japanese Encephalitis (JE) . Histocompatibility Committee. 2. What . p. roblem will the proposal solve? . Incorrectly entered HLA data in UNet is a patient safety risk. E. xample: A23 instead of A32. Creates system inefficiencies . . COVID-19 Consortium Project. IHIW18 Interim Symposium 7. March 26, 2021. Project leaders: Jill A. Hollenbach, Steven J. Mack, Martin Maiers. COVID-19 | HLA & IMMUNOGENETICS CONSORTIUM. A consortium of 130 HLA and immunogenetics-focused academic research scientists, HLA clinical laboratory directors, leaders of international immunogenetics scientific societies, editors of HLA and immunogenetics-focused journals and commercial vendors of HLA laboratory and bioinformatics products from 21 countries on every continent . Clinical Implementation in CLIA/CAP facility. Shashikant Kulkarni, M.S (Medicine)., Ph.D., FACMG. Associate Professor of Pediatrics, Genetics, Pathology and Immunology. Medical Director of Genomics and Pathology Services. Wawina-Bokalanga T, Vanmechelen B, Lhermitte V, Martí-Carreras J, Vergote V, Koundouno F, et al. Human Diversity of Killer Cell Immunoglobulin-Like Receptors and Human Leukocyte Antigen Class I Alleles and Ebola Virus Disease Outcomes. Emerg Infect Dis. 2021;27(1):76-84. https://doi.org/10.3201/eid2701.202177. . molecules. , . their. . structure. , and . function. .. HLA . system. , 2nd . class. . molecules. , . their. . structure. , and . function. .. HLA . system. , . genetic. background. HLA . typing.