PPT-Introduction To Next Generation Sequencing (NGS) Data Anal

Jenny Wu UCI Genomics High Throughput Facility Outline Goals Practical guide to NGS data processing Bioinformatics in NGS data analysis Basics terminology data file formats general workflow . INTRODUCTION . The most commonly used technology until a few years ago – BAC. WHOLE GENOME SEQUENCING. ADVANTAGES OF WGS. Utility of next – gen sequence reads . The next-generation platforms are effecting a complete paradigm shift, not only in the organization of large-scale data production, but also in the downstream bioinformatics, IT, and LIMS support required for high data utility and correct interpretation.. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Eric T. Weimer, PhD, D(ABMLI). CONFLICT OF . INTEREST. I . have financial relationship(s) with: . Advisory Board. , Illumina. NGS Implementation at UNC. Received ASHI accreditation in . August . 2015. Eric T. Weimer, Ph.D., D(ABMLI). Assistant Professor, Pathology and Laboratory Medicine. Associate Director, Clinical Flow Cytometry, HLA, and Immunology . Laboratories. CONFLICT OF . INTEREST. I . have financial relationship(s) with: . Ion . Mandoiu. Computer Science and Engineering Department. University of Connecticut. Outline. Background on high-throughput sequencing. Identification of tumor-specific . epitopes. Estimation of gene and . An Anal Fistula ( Bhagandar / Fistula in ano ) is a small channel that develops between the end of the bowel and the skin around the anus. It often is the result of a previous or current anal abscess. Fistula has an area with two openings - Internal opening into the rectum or anal canal and External opening through the skin of the rumps. Cynthia N Perry, PhD. Assistant Academic Dean of Admissions. Assistant Professor, Molecular Medicine. Department of Medical Education. Foster School of Medicine. https://. youtu.be. /KiQgrK3tge8. Genomics and Healthcare. Clinical Implementation in CLIA/CAP facility. Shashikant Kulkarni, M.S (Medicine)., Ph.D., FACMG. Associate Professor of Pediatrics, Genetics, Pathology and Immunology. Medical Director of Genomics and Pathology Services. Biological Chemistry 3. DNA sequencing. Sequencing. 1) DNA sequencing: . Maxam. -Gilbert method. http://. en.wikipedia.org/wiki/Maxam%E2%80%93Gilbert_sequencing. 2) DNA sequencing: Sanger method. http://www.bio.davidson.edu/Courses/Bio111/seq.html. Vladimir Teif. Intro to NGS analysis. Proficio. course 2020. NGS techniques vs. NGS applications. NGS techniques. : . how. to sequence DNA (or RNA). (covered in lecture 1; funny recap in this video. -- A Whirlwind tour. Presented by Daniel Winester, Geodesist, NGS Gravimetry Representative to the Geophysics Committee of U.S. National Section of PAIGH since 2002. In the past few decades NGS’ gravity work has concentrated on. - An overview of the technology. - Analysis software. - Fetal fraction (FF). - What is fetal fraction?. - Why fetal fraction is used. - Dynamic fetal fraction. Module 6:. NGS and Fetal . F. raction. Vojtěch Bystrý. CEITEC Bioinformatics Core Facility. CEITEC research areas. FI MUNI Bioinformatics Seminar. 2. Bioinformatics CF mission . Primary NGS analysis. S. econdary standard NGS analysis. Custom (project specific) analysis. in the Era of Precision Medicine. JSCO 50. th. Annual Meeting. Yokohama, Japan. October 27, 2012. Mark E. Sobel MD, PhD. Executive Officer. American Society for Investigative Pathology. mesobel@asip.org.