PPT-The Past, Present, and Future of DNA Sequencing

Craig A Praul Co Director Genomics Core Facility Huck Institutes of the Life Sciences Penn State University A very short history of DNA sequencing I started from the conviction that if different DNA species exhibited . Shubha Ghosh. Vilas Research Fellow & Professor of Law. University of Wisconsin Law School. Varieties of TK. October 24, 2011. Ghosh--Past, Present, Future of TK. 2. Commons. Distributive and Intergenerational Justice. Learning Objectives. Recap how DNA probes and DNA hybridisation is used to locate specific genes.. Learn how the exact order of nucleotides on a strand of DNA can be determined.. Learn how restriction mapping can be used to determine nucleotide sequences.. MTL TPTL MITL MTLF TPTLF MTL+Past MITL+Past interval-basedsemantics MTL TPTL MITL MTLF TPTLF MTL+Past MITL+Past pointwisesemantics Figure1:Summaryofourexpressivenessresults(dashededgesindicatefolkresu Dan . Russell. The past, present, and future of DNA . sequencing*. Dan . Russell. *DNA sequencing:. D. etermining the number and order of nucleotides that make up a given molecule of DNA.. (Relevant) Trivia. INTRODUCTION . The most commonly used technology until a few years ago – BAC. WHOLE GENOME SEQUENCING. ADVANTAGES OF WGS. Utility of next – gen sequence reads . The next-generation platforms are effecting a complete paradigm shift, not only in the organization of large-scale data production, but also in the downstream bioinformatics, IT, and LIMS support required for high data utility and correct interpretation.. MOLECULAR BIOLOGY TECHNIQUES II.. Polymerase Chain Reacton – PCR. DNA sequencing. Amplification of specific DNA fragments. MOLECULAR BIOLOGY – PCR. Cloning and/ or isolation from a genomic library . Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. Ryan Hennig. Hadoop Platform Team. ABOUT ME. RYAN HENNIG. Born and raised in Seattle, WA. Studied Computer Science at University of Washington in Seattle. Worked on Microsoft SQL Server 2006 – 2012.  . Nearly . all modern DNA sequencing procedures require a concentrated amount of . single-stranded . DNA known as a . template. . A template is simply a piece of DNA of . sufficient . length and quality to allow for its sequencing (most sequencing methods . How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. Goals:. Review central dogma and limits of DNA. Understand history and recent advances in sequencing. Understand the process (sequencing by synthesis) used to generate data in this module. What is DNA?. Knowing how many genes determine a phenotype (Mendelian and/or QTL analysis), and where the genes are located (linkage mapping) is a first step in understanding the genetic basis of a phenotype . A . Adapted . from:. http. ://. ase.tufts.edu/chemistry/walt/sepa/geneticsofrace.html. Uploaded: January 8, 2017. Genetics of Race. Lesson 1: . Introduction. Goals:. Introduce module topic . Provide necessary background. The Sanger method. DNA sequencing is the primary method for gene and protein characterization. . (protein sequence can be predicted from DNA sequence). . https://. www.illumina.com. /techniques/sequencing/.