PPT-Lesson: Sequencing by synthesis

Goals Review central dogma and limits of DNA Understand history and recent advances in sequencing Understand the process sequencing by synthesis used to generate data in this module What is DNA. Learning Objectives. Recap how DNA probes and DNA hybridisation is used to locate specific genes.. Learn how the exact order of nucleotides on a strand of DNA can be determined.. Learn how restriction mapping can be used to determine nucleotide sequences.. The Mitten. Essential Question. How do readers use sequencing to understand and retell the text?. 2. Topic & Grade Level. Topic. Sequencing events & building a timeline to assist students in recalling the events of a story.. Lenka Veselovská. Laboratory of Developmental Biology and Genomics . Next Generation Sequencing (NGS) . M. odern high-throughput DNA sequencing technologies. parallel, rapid . Decreasing price, time, workflow complexity, error rate. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. Hardison. Genomics . 3_2. 1. 1/20/14. Second generation sequencing. Michael . Metzker. review. (2010) Nature Reviews Genetics . 11. : 31-46. 1/20/14. 2. Two generations of sequencing technology. Feature. Dr. Jennifer Weller. May 2016. Sequencing Technologies. 5/20/2016. Dr. Weller B3 Olympic HS. 1. Topics. Fundamentals of ‘Sequencing by Synthesis’. Sequencing Technologies or ‘Platforms’. Sanger/Capillary sequencing. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. Archival storageA method of retaining information outside of the internal memory of a computer. Molecular digital data storage LuisCeze *, JeffNivala and KarinStraussAbstract Molecular data stora Goals:. Introduce DNA Assembly and Alignment. Practice . rebuilding full sequences from reads. Sequencing by Synthesis Review. Modified PCR “builds” sequence over multiple cycles. Each strand of DNA is amplified into a cluster of identical DNA before sequencing. mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. Hardison. Genomics 3_1. 1. 1/20/14. nucleo. s. ides. A nucleo. t. ide has one or more phosphates attached to the 5’ hydroxyl of the (deoxy)ribose. Building blocks of DNA. 1/20/14. 2. Structure of a dinucleotide. Adapted . from:. http. ://. ase.tufts.edu/chemistry/walt/sepa/geneticsofrace.html. Uploaded: January 8, 2017. Genetics of Race. Lesson 1: . Introduction. Goals:. Introduce module topic . Provide necessary background. DNA Replication Review. Enzymes open . double stranded DNA. Origin of replication. Replication fork. DNA replication in 5’ to 3’ direction. Leading and Lagging Strands. RNA primers. DNA polymerase. DNA storage overall structure. Encode/ECC code. Basic operations: synthesis and sequencing. Some existing works for DNA storage:. DNA archive storage system. OligoArchive. for database system. Puddle: A Dynamic, Error-Correcting, Full-Stack Microfluidics Platform.