PPT-16.6 – Locating and Sequencing Genes

Learning Objectives Recap how DNA probes and DNA hybridisation is used to locate specific genes Learn how the exact order of nucleotides on a strand of DNA can be determined Learn how restriction mapping can be used to determine nucleotide sequences. Introduction and Purpose 3 57364573615734757347573473DUHQWDO57347ULJKWV57347PXVW57347EH57347SURWHFWHG 57365573615734757347EVHQW57347SDUHQWV57347VKRXOG57347EH57347LGHQWLILHG5735957347ORFDW HG5735957347DQG57347FRQWDFWHG57347DV57347TXLFNO57347DV57347S Dan . Russell. The past, present, and future of DNA . sequencing*. Dan . Russell. *DNA sequencing:. D. etermining the number and order of nucleotides that make up a given molecule of DNA.. (Relevant) Trivia. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . Stefano . Lise. Bioinformatics & Statistical Genetics (BSG) Core. The . Wellcome. Trust Centre for Human Genetics (WTCHG), Oxford. Email: stefano@well.ox.ac.uk. Outline. Human genetic variation in health and disease. BIOS . 6660 . Hung-Chun (James) Yu. Shaikh Lab. 04/28/2014. Human Genetic . Diseases. Penetrance vs . F. requency. Kaiser J. . S. cience. (2012) 338:1016-1017.. Human Genetic . Diseases. Complex Disorder. Essential . Questions. What are earthquake magnitude and intensity and how are they measured?. Why are data from at least three seismic stations needed to locate an earthquake’s epicenter?. Where are Earth’s seismic belts?. (very) large datasets. 5/23/17. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. Referencing and Repeatability. "Referencing" is a dual process of positioning the workpiece relative to the . workholder. , and the . workholder. relative to the cutting tool. .. If a part is incorrectly placed in a . A second step is determining the sequence of the gene, or genes, determining the phenotype and understanding how the expression of the genes is regulated at the transcriptional level  . Subsequent steps involve analysis of post-transcriptional events, understanding how the genes fit into metabolic pathways and how these pathways interact with the environment . (very) large datasets. 5/24/18. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. AMEC Environment & Infrastructure. November 2013. Ground Disturbance. Lessons . Learned. AMEC Safety . Rule and Procedures. . Utility Locates, Technology and Limitations. Training. Emergency Response. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. Laboratory of Personalized Genomic Medicine . Peter L. Nagy M.D., Ph.D. Assistant Professor. Associate Director, Laboratory of Personalized Genomic Medicine. Department of Pathology and Cell Biology. . Knowing how many genes determine a phenotype (Mendelian and/or QTL analysis), and where the genes are located (linkage mapping) is a first step in understanding the genetic basis of a phenotype . A second step is determining the sequence of the gene (or genes).