PPT-Clinical next-gen sequencing at CUMC

Author : delilah | Published Date : 2022-06-18

Laboratory of Personalized Genomic Medicine Peter L Nagy MD PhD Assistant Professor Associate Director Laboratory of Personalized Genomic Medicine Department of

Presentation Embed Code

Download Presentation

Download Presentation The PPT/PDF document "Clinical next-gen sequencing at CUMC" is the property of its rightful owner. Permission is granted to download and print the materials on this website for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.

Clinical next-gen sequencing at CUMC: Transcript

Laboratory of Personalized Genomic Medicine Peter L Nagy MD PhD Assistant Professor Associate Director Laboratory of Personalized Genomic Medicine Department of Pathology and Cell Biology. . Content by . Marc . Salit. & Justin . Zook. (NIST). and. Liz Mansfield & . Zivana. . Tezak. (FDA). As delivered by Daryl Thomas. NIST Perspective on standards for genome measurement assurance. Dan . Russell. Overview. Prologue: Assembly and Finishing. The Past: Sanger. The Present: Next-Gen (454, . Illumina. , …). The Future: ? (. Nanopore. , . MinION. , Single-molecule). Overview. Prologue: Assembly and Finishing. Dan . Russell. The past, present, and future of DNA . sequencing*. Dan . Russell. *DNA sequencing:. D. etermining the number and order of nucleotides that make up a given molecule of DNA.. (Relevant) Trivia. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . Mendelian. Inheritance. Genetics 202. Jon Bernstein. Department of Pediatrics. October 1. st. , 2015. Learning Goals. Understand that . different molecular . testing methodologies detect different types of mutation. May 31, 2017. Office of . Student Services. Important . New Student . Info: . CUMC Exchange . E-mail, Matriculation. , Registration, CUID Cards, . Student . Status, Boat . Cruise. Student Affairs Offices. 19 May 2014. Jim Evans MD, . Ph.D. University of North Carolina at Chapel Hill. Accomplishments, Challenges and Opportunities. Green & . Guyer. . Nature; 470:204.2011. Medical Science = Clinical Care. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. Adapted . from:. http. ://. ase.tufts.edu/chemistry/walt/sepa/geneticsofrace.html. Uploaded: January 8, 2017. Genetics of Race. Lesson 1: . Introduction. Goals:. Introduce module topic . Provide necessary background. ? . An . introduction to human . genomics. Jacques Fellay. EPFL School of Life Sciences. Swiss Institute of Bioinformatics. Lausanne, Switzerland. What is . the genome?. “It's . a . shop manual. , with an incredibly detailed blueprint for building every human cell.. in Advanced Solid Tumors. EDRN Biomarker Development Lab. Arul M. Chinnaiyan, M.D., Ph.D.. American Cancer Society Research Professor. Howard Hughes Medical Institute. Sequencing. Buccal swab . or. Blood. Clinical Implementation in CLIA/CAP facility. Shashikant Kulkarni, M.S (Medicine)., Ph.D., FACMG. Associate Professor of Pediatrics, Genetics, Pathology and Immunology. Medical Director of Genomics and Pathology Services. Departiment. of Diagnostics and Laboratory Medicine. A. . Gemelli. – School of Medicine, Rome. Next. generation . techniques. for . biomarker. . identification. I. ssues regarding . BRCA1/2. Sanger .

Download Document

Here is the link to download the presentation.
"Clinical next-gen sequencing at CUMC"The content belongs to its owner. You may download and print it for personal use, without modification, and keep all copyright notices. By downloading, you agree to these terms.