PPT-MOLECULAR BIOLOGY – PCR, sequencing, Genomics

MOLECULAR BIOLOGY TECHNIQUES II Polymerase Chain Reacton PCR DNA sequencing Amplification of specific DNA fragments MOLECULAR BIOLOGY PCR Cloning and or isolation from a genomic library . 2012 HHMI Summer Workshop for High School Science . Teachers. The Genomics of . S.cerevisiae. GOALS. Introduction to the Genomics of Yeast. Sequencing Technologies and how they are evolving. Introduction to Systems Biology and modern Yeast Genetics. Facts & . Achievements. "Biochemistry brings molecular science to life". . 1. 2. Introduction. Education and Research – organizational . structure. Educational offer. National . and international visibility. DNA sequencing. Why? . – Identifies Organisms. How? – . Dideoxy Sequencing. Introduces Biological Machines. Where? – . Universities, MBL. Societal Impact? . – Forensics, Insurance. DNA detective. MOLECULAR BIOLOGY TECHNIQUES II.. Polymerase Chain Reacton – PCR. DNA sequencing. Amplification of specific DNA fragments. MOLECULAR BIOLOGY – PCR. Cloning and/ or isolation from a genomic library . Alberto Catalano. email: . catalano.alberto@gmail.com. Outline of topics. Molecular biology refresher. Molecular biology in haematology. Quality control issues. Laboratory layout and equipment. Case studies. How cells are organized and work. Genetics: The Information. How the machinery is encoded in the genome and how the genome is inherited. Course Content. DNA sequencing and related approaches. Sample Preparation. Department of Biochemistry and Molecular Biology Today Facts & Achievements "Biochemistry brings molecular science to life" 1 2 Introduction Education and Research – organizational structure Hardison. Genomics 2_1. 3/1/15. 1. Y Chromosome. 3 billion bp = 3 Gb. Chr1 247 Mb. Chr12 132 Mb. Chr22 50 Mb. A human . genome (. male). The . genome. is . all the DNA. in a cell.. All the DNA on all the chromosomes. Next-generation Sequencing. Work flow of conventional versus second-generation sequencing. (a) With high-throughput shotgun Sanger sequencing, genomic DNA is fragmented, then cloned to a plasmid vector and used to transform E. coli. For each sequencing reaction, a single bacterial colony is picked and plasmid DNA isolated. Each cycle sequencing reaction takes place within a microliter-scale volume, generating a ladder of ddNTP-terminated, dye-labeled products, which are subjected to high-resolution electrophoretic separation within one of 96 or 384 capillaries in one run of a sequencing instrument. As fluorescently labeled fragments of discrete sizes pass a detector, the four-channel emission spectrum is used to generate a sequencing trace. . Real-Time PCR is a specialized technique that allows a PCR reaction to be visualized “in real time” as the reaction progresses.. This enables researchers to quantify the amount of DNA in the sample at the start of the reaction!. Institute of Medical S ciences (IMS) Banaras Hindu University (BHU) Varanasi - 221005 Junior Research Fellow Applications are invited for the one post of the Junior Research Fellow ( JRF ) with a s Century: Mendelian Principles are extended and the Chromosomal Theory of Except for his early adult years, Mendel e research program. who had performed similar experiments to his, arrived at the sa Abdul Karim Sesay . Genomics Strategic Core Platform, MRC Unit The Gambia @ LSHTM. Wellcome. Trust - Bloomsbury Centre for Global Health. Research Scientific Meeting. 17th March 2022. Genomics. DNA sequencing and the capacity to investigate the genome of host and pathogen populations have become an essential part of biomedical research, unlocking unique information about why some . 14/11, ore 14-16, edificio Q. 20/11, ore 11-13. , . edificio Q. 21/11, ore 14-16, . edificio . Q. 22/11, ore 9-11, edificio Q. 27/11, ore 11-13, aula Z. 29/11, ore 9-11, aula Z. CALENDARIO DELLE LEZIONI - DICEMBRE.