PPT-MOLECULAR BIOLOGY – PCR, sequencing, Genomics

MOLECULAR BIOLOGY TECHNIQUES II Polymerase Chain Reacton PCR DNA sequencing Amplification of specific DNA fragments MOLECULAR BIOLOGY PCR Cloning and or isolation from a genomic library . DNA sequencing. Why? . – Identifies Organisms. How? – . Dideoxy Sequencing. Introduces Biological Machines. Where? – . Universities, MBL. Societal Impact? . – Forensics, Insurance. DNA detective. MOLECULAR BIOLOGY TECHNIQUES II.. Polymerase Chain Reacton – PCR. DNA sequencing. Amplification of specific DNA fragments. MOLECULAR BIOLOGY – PCR. Cloning and/ or isolation from a genomic library . How cells are organized and work. Genetics: The Information. How the machinery is encoded in the genome and how the genome is inherited. Course Content. DNA sequencing and related approaches. Sample Preparation. Department of Biochemistry and Molecular Biology Today Facts & Achievements "Biochemistry brings molecular science to life" 1 2 Introduction Education and Research – organizational structure Human Chromosomes Identification by G-Banding. Karyotyping. MOLECULAR BIOLOGY. Experiment Objectives. Preparing, Staining and Observing G-banding human chromosomes. Develop an understanding of karyotyping and the association of various chromosomal abnormalities to diseases.. , a technique used to make numerous copies of a specific segment of . DNA.  quickly and accurately. The polymerase . chain reaction.  enables investigators to obtain the large quantities of DNA that are required for various experiments and procedures in . Hardison. Genomics 2_1. 3/1/15. 1. Y Chromosome. 3 billion bp = 3 Gb. Chr1 247 Mb. Chr12 132 Mb. Chr22 50 Mb. A human . genome (. male). The . genome. is . all the DNA. in a cell.. All the DNA on all the chromosomes. “. User facility. providing high-throughput DNA sequencing & analysis in support of DOE missions in alternative . energy. , . carbon cycling . & . bioremediation. .”. Opened in 1999. ~240 UC Employees. Alan . Christoffels. South African Medical Research Council Bioinformatics Unit, South African National Bioinformatics Institute, University of the Western Cape, South Africa, Secretariat: Public Health Alliance for Genomic Epidemiology. Genomics and Society Nature (2014) “ …disorders not readily explained by standard tests can sometimes be diagnosed through genome sequencing and analysis.” The Origin of “Genomics& ;. . the. . study of . biology. at the . molecular. level.. Molecular biology. ;. . the. . study of . gene . structure and functions at the . molecular level. . to understand the molecular basis of hereditary, genetic variation, and the expression patterns of genes. . Biochemistry examines the rudimentary chemistry of life. Molecular biology studies the complex interactions among biological molecules. ASBMB publishes three prestigious research journals. ASBMB's journals publish original research in the fields of microbiology, molecular genetics, RNA-related research, proteomics, genomics, transcription, peptides, cell signaling, . Abdul Karim Sesay . Genomics Strategic Core Platform, MRC Unit The Gambia @ LSHTM. Wellcome. Trust - Bloomsbury Centre for Global Health. Research Scientific Meeting. 17th March 2022. Genomics. DNA sequencing and the capacity to investigate the genome of host and pathogen populations have become an essential part of biomedical research, unlocking unique information about why some . Ravi Madduri. madduri@anl.gov. Joint work with Paul . Davé. , Lukasz . Lacinski. , Alex Rodriguez, . Dinanath. . Sulakhe. , Ryan Chard and Ian Foster. Globus Genomics is developed, operated, and supported by researchers, developers, and .