PPT-Course Expectations Sequencing technology and

very large datasets 52317 Goals for the course Understand how nextgeneration sequencing technologies are used in biomedical research Learn how to use publicly available databaseswebsites to find specific information about genes. Introduce precise claims distinguish the c laims from alternate or opposing claims and create an organization that establishes clear relationships among the claims counterclaims reasons and evidence b Develop claims and counterclaims fairly supplyin Dan . Russell. Overview. Prologue: Assembly and Finishing. The Past: Sanger. The Present: Next-Gen (454, . Illumina. , …). The Future: ? (. Nanopore. , . MinION. , Single-molecule). Overview. Prologue: Assembly and Finishing. Dan . Russell. The past, present, and future of DNA . sequencing*. Dan . Russell. *DNA sequencing:. D. etermining the number and order of nucleotides that make up a given molecule of DNA.. (Relevant) Trivia. Craig A. . Praul. Co- Director . Genomics Core Facility. Huck Institutes of the Life Sciences. Penn State University. A very short history of DNA sequencing. I started from the conviction that, if different DNA species exhibited . Gene Expression . Next-Generation . DNA Sequencing Technology. Stuart M. Brown, Ph.D. This Lecture. Learning Objectives. Illumina Sequencing Technology: . shotgun, amplicon, selected. Quality Scores. (very) large datasets. 5/24/18. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. :. Computational Analysis of the Current State, Bottlenecks and Future Directions. Damla Senol. 1. , Jeremie . Kim. 1,3. , . Saugata Ghose. 1. , Can Alkan. 2. and Onur Mutlu. 3,1. 1 . Department of Electrical and Computer Engineering, Carnegie Mellon University, Pittsburgh, PA, USA. :. Computational Analysis of the Current State, Bottlenecks and Future Directions. Damla Senol. 1. , Jeremie Kim. 1,3. , Saugata Ghose. 1. , Can Alkan. 2. and Onur . Mutlu. 3,1. Nanopore Sequencing. mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. DNA polymerase (copy DNA), restriction endonucleases (cut DNA), ligases (join DNA). DNA cloning – vector (plasmid, BAC), PCR. genome mapping. relative locations of genes are established by following inheritance patterns. and Industrial Research (BCSIR), Dhaka, Bangladesh 14 Pyrosequencing-An Alternative to Traditional Sanger Sequencing Fakruddin and Abhijit Chowdhury Institute of Food Science and Technology (IFST), B and Accurate Assembly Polishing Algorithm. Can Firtina. 1. , Jeremie S. Kim. 1,2. , Mohammed Alser. 1. , . Damla. . Senol. Cali. 2. , A. . Ercument. Cicek. 3. ,. Can Alkan. 3. , and . Onur. Mutlu. Using a genetic condition as an examples, describe a sequencing technology and how it works to a non-specialist healthcare professional. . Describe a DNA sequencing technology which we may expect to see being used in genomics laboratories in the future. .