PPT-Human Sequencing

Author : natalia-silvester | Published Date : 2016-07-24

Stefano Lise Bioinformatics amp Statistical Genetics BSG Core The Wellcome Trust Centre for Human Genetics WTCHG Oxford Email stefanowelloxacuk Outline Human genetic

Presentation Embed Code

Download Presentation

Download Presentation The PPT/PDF document "Human Sequencing" is the property of its rightful owner. Permission is granted to download and print the materials on this website for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.

Human Sequencing: Transcript

Stefano Lise Bioinformatics amp Statistical Genetics BSG Core The Wellcome Trust Centre for Human Genetics WTCHG Oxford Email stefanowelloxacuk Outline Human genetic variation in health and disease. Last lecture summary. recombinant DNA technology. DNA polymerase (copy DNA), restriction endonucleases (cut DNA), ligases (join DNA). DNA cloning – vector (plasmid, BAC), PCR. genome mapping. relative locations of genes are established by following inheritance patterns. DNA sequencing. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. Dan . Russell. The past, present, and future of DNA . sequencing*. Dan . Russell. *DNA sequencing:. D. etermining the number and order of nucleotides that make up a given molecule of DNA.. (Relevant) Trivia. DNA sequencing. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. Venessa. Williams. Hai Tran. Myles Everett. Alex . Detlefsen. Bio 1510-A Group A39. September 13, 2012. Introduction. Little known about genome sequences of Africans. Common ancestors. 1000 Genomes Project used new technology to view whole genome sequences. Biotechnology Course. Spring 2010. What is gel electrophoresis?. -. -. -. -. -. -. -. . . . . . . wells. DNA (-). Loading . Buffer. pcr. products. . STAIN. DNA Sequencing. DNA Sequencing: Basic Principle. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. Goals:. Review central dogma and limits of DNA. Understand history and recent advances in sequencing. Understand the process (sequencing by synthesis) used to generate data in this module. What is DNA?. Hierarchical genome shotgun HGS – Human Genome Project. “map first, sequence second. ”. clone-by-clone … cloning is performed twice (BAC, plasmid). Sequencing strategies. Whole genome shotgun WGS – Celera. Modified from Dan Russell. (Relevant) Trivia. How many base pairs (bp) are there in a human genome?. How many protein coding genes are in the Human genome. How much did it cost to sequence the first human genome?. DNA - the basics. Drew Berry – DNA animations. http://. www.youtube.com. /. watch?v. =. WFCvkkDSfIU&index. =4&list=PL9CBBEA5A85DBCDEF. Organisation of DNA. DNA is packed in Chromosomes . Karyotype: chromosome set of a species . . In the paper, researchers describe the final product of the Human Genome Project, which was the 13year effort to read the information encoded in the human chromosomes that reached its culmination i T – Its History and Advancements into New Research and Technology Jutta Marzillier , Ph.D Lehigh University Biological Sciences September 5 th , 2014 Objectives Techniques that enabled genome sequ Igor Ulitsky. “the . branch of genetics that studies organisms in terms of their genomes (their full DNA sequences. )”. Computational genomics in TAU. Ron Shamir’s lab – focus on gene expression and regulatory networks.

Download Document

Here is the link to download the presentation.
"Human Sequencing"The content belongs to its owner. You may download and print it for personal use, without modification, and keep all copyright notices. By downloading, you agree to these terms.