PPT-BIO00058M: Sequence Analysis

Lecture 3 Highthroughput sequencing Part 2 Kanchon Dasmahapatra kanchondasmahapatrayorkacuk Room J101 L3 W3 Learning objectives Have a understanding of resequencing genomes Aligning to a reference genome. Textco BioSoftware (formerly Textco, Inc.), has been developing high quality productivity tools for molecular biologists for over 25 years. Our unwavering commitment to customer service, and our focus on quality has generated a loyal customer following. Since 1984, we have provided solutions to scientists who are breaking new ground in genetic engineering, basic biology research, drug development, and biotechnology - at academic, government, and corporate institutions in more than 50 countries worldwide. Lian Yang. 2. ; . Baozhen Shan. 1. ; . Bin Ma. 2. 1. Bioinformatics Solutions Inc, Canada . 2. University of Waterloo, Canada. Problem. . Complete protein sequence coverage. antibody confirmation. Scott Walmsley, PhD. Research Instructor, Department Pharmaceutical Sciences. Skaggs School of Pharmacy. Outline . What is and why perform Multiple . S. equence . A. lignment (MSA)?. Pre-requisite knowledge. A (soon to be outdated) Tutorial. A Brief History of Sequencing and Gene Expression. Limitations of Sanger Sequencing. Low throughput. Inconsistent base quality. Expensive. Not quantitative. Frederick “. Utah State University – Spring . 2012. STAT 5570: Statistical Bioinformatics. Notes 6.1. 2. References. Chapters 2 & 7 of Biological Sequence Analysis (Durbin et al., 2001). . 3. Review. Genes are:. BIOST 2055. 04/06/2015. Last Lecture . Genome-wide association study has identified thousands of disease-associated loci. Large consortium performs meta-analysis to further increase the sample size (power) to detect additional loci. Utah State University – Spring . 2012. STAT 5570: Statistical Bioinformatics. Notes 6.1. 2. References. Chapters 2 & 7 of Biological Sequence Analysis (Durbin et al., 2001). . 3. Review. Genes are:. A. ll?. Using SystemVerilog UVM Sequences for Fun and Profit. by. Rich Edelman and Raghu Ardeishar. Verification Technologists. Mentor Graphics. Questa Verification Platform. thefreedictionary.com/fairest . Sequence, Sequence on the Wall, Who’s the Fairest of Them A ll? Using SystemVerilog UVM Sequences for Fun and Profit by Rich Edelman and Raghu Ardeishar Verification Technologists Mentor Graphics Questa Verification Platform Alshahrani. CS6800. Statistical Background : HMMs.. What is DNA Sequence. . How to get DNA Sequence.. DNA Sequence formats.. Analysis methods and tools.. What is next ?. HMMs. Hidden Markov Model (. Section 1: Introduction and biological databases.. Section 2: Sequence alignment.. Section 3: Gene and promoter prediction.. Section 4: Molecular phylogenetics.. Section 5: Structural Bioinformatics. - Overview -. Why gene expression analysis?. Quantification of mRNA transcript abundance. High specificity, +/- high through-put. Requires sequence knowledge. Considerations. Experimental question. Species limitations . Lian Yang. 2. ; . Baozhen Shan. 1. ; . Bin Ma. 2. 1. Bioinformatics Solutions Inc, Canada . 2. University of Waterloo, Canada. Problem. . Complete protein sequence coverage. antibody confirmation. 19 July 2011. Richard H. Scheuermann, Ph.D.. Department of Pathology. U.T. Southwestern Medical Center. Outline. Brief o. verview of NIAID-Sponsored Influenza Research Database (IRD). Comprehensive integrated database.